Dispersive liquid―liquid microextraction using functionalized Mg(OH)2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS
We report a dispersive liquid-liquid microextraction (LLME) method using surface modified Mg(OH)(2) nanoparticles (NPs) with oleic acid (OA) as hydrophobic affinity probes for the extraction and preconcentration of hydrophobic biomolecules prior to MALDI MS analysis. OA-capped Mg(OH)(2) NPs were cha...
Gespeichert in:
| Veröffentlicht in: | Analyst (London) 2012-10, Vol.137 (19), p.4490-4496 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4496 |
|---|---|
| container_issue | 19 |
| container_start_page | 4490 |
| container_title | Analyst (London) |
| container_volume | 137 |
| creator | KUMAR KAILASA, Suresh WU, Hui-Fen |
| description | We report a dispersive liquid-liquid microextraction (LLME) method using surface modified Mg(OH)(2) nanoparticles (NPs) with oleic acid (OA) as hydrophobic affinity probes for the extraction and preconcentration of hydrophobic biomolecules prior to MALDI MS analysis. OA-capped Mg(OH)(2) NPs were characterized by FT-IR, SEM and TEM. The functionalized Mg(OH)(2) NPs are well dispersed in toluene with an average diameter of |
| doi_str_mv | 10.1039/c2an35788g |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1037242447</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1037242447</sourcerecordid><originalsourceid>FETCH-LOGICAL-c232t-3075ae0e93f20cfc0f5119eeae3ff60be58bd166126eda4b267c995850ecef2a3</originalsourceid><addsrcrecordid>eNpV0c9u1DAQBnALUdGlcOEB0FyQSqWAY8f5c6xaaCvtUiTgvHKc8e6gbLL1JEA48RI8DW_Dk9RlFypO9lg_fRr5E-JZKl-lUlevnbKdNkVZrh6IWarzLDFGlQ_FTEqpE5Wb7FA8Zv4cx1Qa-UgcKlWWUkk5E7_OibcYmL4gtHQzUvP7x8_dBTbkQo_fhmDdQH0HI1O3Aj92f0bb0ndsYLE6vr58qeDde4avNKyhb5EcWBcDLMN6akK_Xff13Zv31NEwwTb0NTL4PsCwRrAxa2Ji6P1_PrIBqWOgDuq4AwayUE-wOJ2fX8HiwxNx4G3L-HR_HolPb998PLtM5tcXV2en88QprYZEy8JYlFhpr6TzTnqTphWiRe19Lms0Zd2keZ6qHBub1SovXFWZ0kh06JXVR-J4lxsXuhmRh-WG2GHb2g77kZexhEJlKsuKSE92NP4cc0C_3Aba2DBFdOeq5X1ZET_f5471Bpt_9G87EbzYA8vOtj7YzhHfu1zLqpBa3wJ3AqGb</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1037242447</pqid></control><display><type>article</type><title>Dispersive liquid―liquid microextraction using functionalized Mg(OH)2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS</title><source>Royal Society of Chemistry Journals Archive (1841-2007)</source><source>MEDLINE</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>KUMAR KAILASA, Suresh ; WU, Hui-Fen</creator><creatorcontrib>KUMAR KAILASA, Suresh ; WU, Hui-Fen</creatorcontrib><description>We report a dispersive liquid-liquid microextraction (LLME) method using surface modified Mg(OH)(2) nanoparticles (NPs) with oleic acid (OA) as hydrophobic affinity probes for the extraction and preconcentration of hydrophobic biomolecules prior to MALDI MS analysis. OA-capped Mg(OH)(2) NPs were characterized by FT-IR, SEM and TEM. The functionalized Mg(OH)(2) NPs are well dispersed in toluene with an average diameter of <35 nm. Under the optimum conditions, the method showed good linearity in the ranges of 10-400 nM and 0. 1-2.0 μM for valinomycin and gramicidin D, respectively. The limits of detection were in the region of 2.0 nM and 24 nM for valinomycin and gramicidin D, respectively. Moreover, the OA-capped Mg(OH)(2) NPs-assisted LLME method was successfully used for the rapid extraction/preconcentration of hydrophobic proteins in bacteria (Escherichia coli and Bacillus subtilis) prior to their identification by MALDI MS.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c2an35788g</identifier><identifier>PMID: 22880200</identifier><identifier>CODEN: ANALAO</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Affinity Labels - chemistry ; Analytical chemistry ; Bacteria - metabolism ; Bacterial Proteins - analysis ; Bacterial Proteins - isolation & purification ; Chemistry ; Exact sciences and technology ; Hydrophobic and Hydrophilic Interactions ; Liquid Phase Microextraction ; Magnesium Hydroxide - chemistry ; Nanoparticles - chemistry ; Oleic Acid - chemistry ; Spectrometric and optical methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><ispartof>Analyst (London), 2012-10, Vol.137 (19), p.4490-4496</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c232t-3075ae0e93f20cfc0f5119eeae3ff60be58bd166126eda4b267c995850ecef2a3</citedby><cites>FETCH-LOGICAL-c232t-3075ae0e93f20cfc0f5119eeae3ff60be58bd166126eda4b267c995850ecef2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2818,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26309703$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22880200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KUMAR KAILASA, Suresh</creatorcontrib><creatorcontrib>WU, Hui-Fen</creatorcontrib><title>Dispersive liquid―liquid microextraction using functionalized Mg(OH)2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>We report a dispersive liquid-liquid microextraction (LLME) method using surface modified Mg(OH)(2) nanoparticles (NPs) with oleic acid (OA) as hydrophobic affinity probes for the extraction and preconcentration of hydrophobic biomolecules prior to MALDI MS analysis. OA-capped Mg(OH)(2) NPs were characterized by FT-IR, SEM and TEM. The functionalized Mg(OH)(2) NPs are well dispersed in toluene with an average diameter of <35 nm. Under the optimum conditions, the method showed good linearity in the ranges of 10-400 nM and 0. 1-2.0 μM for valinomycin and gramicidin D, respectively. The limits of detection were in the region of 2.0 nM and 24 nM for valinomycin and gramicidin D, respectively. Moreover, the OA-capped Mg(OH)(2) NPs-assisted LLME method was successfully used for the rapid extraction/preconcentration of hydrophobic proteins in bacteria (Escherichia coli and Bacillus subtilis) prior to their identification by MALDI MS.</description><subject>Affinity Labels - chemistry</subject><subject>Analytical chemistry</subject><subject>Bacteria - metabolism</subject><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Liquid Phase Microextraction</subject><subject>Magnesium Hydroxide - chemistry</subject><subject>Nanoparticles - chemistry</subject><subject>Oleic Acid - chemistry</subject><subject>Spectrometric and optical methods</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpV0c9u1DAQBnALUdGlcOEB0FyQSqWAY8f5c6xaaCvtUiTgvHKc8e6gbLL1JEA48RI8DW_Dk9RlFypO9lg_fRr5E-JZKl-lUlevnbKdNkVZrh6IWarzLDFGlQ_FTEqpE5Wb7FA8Zv4cx1Qa-UgcKlWWUkk5E7_OibcYmL4gtHQzUvP7x8_dBTbkQo_fhmDdQH0HI1O3Aj92f0bb0ndsYLE6vr58qeDde4avNKyhb5EcWBcDLMN6akK_Xff13Zv31NEwwTb0NTL4PsCwRrAxa2Ji6P1_PrIBqWOgDuq4AwayUE-wOJ2fX8HiwxNx4G3L-HR_HolPb998PLtM5tcXV2en88QprYZEy8JYlFhpr6TzTnqTphWiRe19Lms0Zd2keZ6qHBub1SovXFWZ0kh06JXVR-J4lxsXuhmRh-WG2GHb2g77kZexhEJlKsuKSE92NP4cc0C_3Aba2DBFdOeq5X1ZET_f5471Bpt_9G87EbzYA8vOtj7YzhHfu1zLqpBa3wJ3AqGb</recordid><startdate>20121007</startdate><enddate>20121007</enddate><creator>KUMAR KAILASA, Suresh</creator><creator>WU, Hui-Fen</creator><general>Royal Society of Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121007</creationdate><title>Dispersive liquid―liquid microextraction using functionalized Mg(OH)2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS</title><author>KUMAR KAILASA, Suresh ; WU, Hui-Fen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c232t-3075ae0e93f20cfc0f5119eeae3ff60be58bd166126eda4b267c995850ecef2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Affinity Labels - chemistry</topic><topic>Analytical chemistry</topic><topic>Bacteria - metabolism</topic><topic>Bacterial Proteins - analysis</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Liquid Phase Microextraction</topic><topic>Magnesium Hydroxide - chemistry</topic><topic>Nanoparticles - chemistry</topic><topic>Oleic Acid - chemistry</topic><topic>Spectrometric and optical methods</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KUMAR KAILASA, Suresh</creatorcontrib><creatorcontrib>WU, Hui-Fen</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KUMAR KAILASA, Suresh</au><au>WU, Hui-Fen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dispersive liquid―liquid microextraction using functionalized Mg(OH)2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2012-10-07</date><risdate>2012</risdate><volume>137</volume><issue>19</issue><spage>4490</spage><epage>4496</epage><pages>4490-4496</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><coden>ANALAO</coden><abstract>We report a dispersive liquid-liquid microextraction (LLME) method using surface modified Mg(OH)(2) nanoparticles (NPs) with oleic acid (OA) as hydrophobic affinity probes for the extraction and preconcentration of hydrophobic biomolecules prior to MALDI MS analysis. OA-capped Mg(OH)(2) NPs were characterized by FT-IR, SEM and TEM. The functionalized Mg(OH)(2) NPs are well dispersed in toluene with an average diameter of <35 nm. Under the optimum conditions, the method showed good linearity in the ranges of 10-400 nM and 0. 1-2.0 μM for valinomycin and gramicidin D, respectively. The limits of detection were in the region of 2.0 nM and 24 nM for valinomycin and gramicidin D, respectively. Moreover, the OA-capped Mg(OH)(2) NPs-assisted LLME method was successfully used for the rapid extraction/preconcentration of hydrophobic proteins in bacteria (Escherichia coli and Bacillus subtilis) prior to their identification by MALDI MS.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>22880200</pmid><doi>10.1039/c2an35788g</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2654 |
| ispartof | Analyst (London), 2012-10, Vol.137 (19), p.4490-4496 |
| issn | 0003-2654 1364-5528 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1037242447 |
| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Affinity Labels - chemistry Analytical chemistry Bacteria - metabolism Bacterial Proteins - analysis Bacterial Proteins - isolation & purification Chemistry Exact sciences and technology Hydrophobic and Hydrophilic Interactions Liquid Phase Microextraction Magnesium Hydroxide - chemistry Nanoparticles - chemistry Oleic Acid - chemistry Spectrometric and optical methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | Dispersive liquid―liquid microextraction using functionalized Mg(OH)2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T04%3A08%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dispersive%20liquid%E2%80%95liquid%20microextraction%20using%20functionalized%20Mg(OH)2%20NPs%20with%20oleic%20acid%20as%20hydrophobic%20affinity%20probes%20for%20the%20analysis%20of%20hydrophobic%20proteins%20in%20bacteria%20by%20MALDI%20MS&rft.jtitle=Analyst%20(London)&rft.au=KUMAR%20KAILASA,%20Suresh&rft.date=2012-10-07&rft.volume=137&rft.issue=19&rft.spage=4490&rft.epage=4496&rft.pages=4490-4496&rft.issn=0003-2654&rft.eissn=1364-5528&rft.coden=ANALAO&rft_id=info:doi/10.1039/c2an35788g&rft_dat=%3Cproquest_cross%3E1037242447%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1037242447&rft_id=info:pmid/22880200&rfr_iscdi=true |