Dispersive liquid―liquid microextraction using functionalized Mg(OH)2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS

We report a dispersive liquid-liquid microextraction (LLME) method using surface modified Mg(OH)(2) nanoparticles (NPs) with oleic acid (OA) as hydrophobic affinity probes for the extraction and preconcentration of hydrophobic biomolecules prior to MALDI MS analysis. OA-capped Mg(OH)(2) NPs were cha...

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Veröffentlicht in:	Analyst (London) 2012-10, Vol.137 (19), p.4490-4496
Hauptverfasser:	KUMAR KAILASA, Suresh, WU, Hui-Fen
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels - chemistry Analytical chemistry Bacteria - metabolism Bacterial Proteins - analysis Bacterial Proteins - isolation & purification Chemistry Exact sciences and technology Hydrophobic and Hydrophilic Interactions Liquid Phase Microextraction Magnesium Hydroxide - chemistry Nanoparticles - chemistry Oleic Acid - chemistry Spectrometric and optical methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Zusammenfassung:	We report a dispersive liquid-liquid microextraction (LLME) method using surface modified Mg(OH)(2) nanoparticles (NPs) with oleic acid (OA) as hydrophobic affinity probes for the extraction and preconcentration of hydrophobic biomolecules prior to MALDI MS analysis. OA-capped Mg(OH)(2) NPs were characterized by FT-IR, SEM and TEM. The functionalized Mg(OH)(2) NPs are well dispersed in toluene with an average diameter of
ISSN:	0003-2654 1364-5528
DOI:	10.1039/c2an35788g