Influence of the Cumulus and Gonadotropins on the Metabolic Profile of Porcine Cumulus–Oocyte Complexes During In Vitro Maturation

The aim of this work was to examine the influence of the cumulus and gonadotropins on the metabolic profile of porcine cumulus oocyte complexes (COCs) during in vitro maturation. Immature COCs were assigned to morphological classes A1 (with a dense cumulus), A2 (with a translucent cumulus), B1 (with...

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Veröffentlicht in:	Reproduction in domestic animals 2012-10, Vol.47 (5), p.856-864
Hauptverfasser:	Alvarez, GM, Dalvit, GC, Cetica, PD
Format:	Artikel
Sprache:	eng
Schlagworte:	amino acids ammonia Ammonia - metabolism Animal reproduction Animal sciences Animals Biological and medical sciences Cell Count Cells, Cultured Cumulus Cells - cytology Cumulus Cells - physiology Female Fertilization in Vitro - veterinary Follicle Stimulating Hormone - pharmacology Fundamental and applied biological sciences. Psychology Glucose - metabolism Glycolysis gonadotropins Gonadotropins - pharmacology Hogs Hormones Lactic Acid - metabolism lipid content Lipids - analysis Lipolysis Luteinizing Hormone - pharmacology Mammalian reproduction. General aspects Metabolism Metabolome oocytes Oocytes - drug effects Oocytes - growth & development Oocytes - metabolism reactive oxygen species Reactive Oxygen Species - analysis Swine Vertebrates: reproduction
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Zusammenfassung:	The aim of this work was to examine the influence of the cumulus and gonadotropins on the metabolic profile of porcine cumulus oocyte complexes (COCs) during in vitro maturation. Immature COCs were assigned to morphological classes A1 (with a dense cumulus), A2 (with a translucent cumulus), B1 (with the corona radiata), B2 (with only some remaining cumulus cells) and matured with or without gonadotropins. Glycolysis and ammonia production were higher in the A class COCs; gonadotropins increased both, especially in the A1 COCs (p
ISSN:	0936-6768 1439-0531
DOI:	10.1111/j.1439-0531.2011.01943.x