Kit formulation for 99mTc-labeling of HYNIC-βAla-Bombesin(7–14)

Bombesin (BBN) is a tetradecapeptide that binds specifically to gastrin-releasing peptide receptors. Several forms of cancer, including lung, prostate, breast, and colon express receptors for bombesin-like peptides. Radiolabeled BBN analogs with a high affinity for these receptors might be used for scintigraphic imaging. Kit formulations for labeling these molecules are important for routine preparation. A freeze-dried kit for labeling HYNIC-βAla-Bombesin(7–14) with technetium-99m was prepared, and its storage stability was evaluated by in vitro and in vivo assays. ► Bombesin kit formulations were successfully prepared. ► High radiochemical purity was achieved for KBBN50 and KBBN100 formulations. ► In vitro assays demonstrated high storage stability for KBBN50. ► Biodistribution and scintigraphic images corroborate with in vitro results.