Kinetics of GDF9 expression in buffalo oocytes during in vitro maturation and their associated development ability

Highlights ► GDF9 expression in buffalo oocytes was found affected by supplements in IVM. ► medium. ► GDF9 mRNA level at 8hr of IVM was crucial for acquiring oocyte development. ► ability. ► Appearance of GDF9 protein in oocytes was found asynchronous with. ► transcription. ► GDF9 protein at 16hr of...

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Veröffentlicht in:	General and comparative endocrinology 2012-09, Vol.178 (3), p.477-484
Hauptverfasser:	Jain, Tripti, Jain, Asit, Kumar, Parveen, Goswami, S.L, De, S, Singh, Dheer, Datta, T.K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals blastocyst Blotting, Western Buffalo buffaloes Buffaloes - embryology Buffaloes - metabolism cell communication Cell Differentiation - genetics Cell Differentiation - physiology Endocrinology & Metabolism Female follicle-stimulating hormone GDF9 genes Growth Differentiation Factor 9 - genetics Growth Differentiation Factor 9 - metabolism Kinetics messenger RNA Oocyte oocytes Oocytes - cytology Oocytes - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger
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Zusammenfassung:	Highlights ► GDF9 expression in buffalo oocytes was found affected by supplements in IVM. ► medium. ► GDF9 mRNA level at 8hr of IVM was crucial for acquiring oocyte development. ► ability. ► Appearance of GDF9 protein in oocytes was found asynchronous with. ► transcription. ► GDF9 protein at 16hr of IVM was significantly correlated with oocyte. ► development. ► This is the first report giving a molecular account of buffalo oocytes maturation.
ISSN:	0016-6480 1095-6840
DOI:	10.1016/j.ygcen.2012.07.001