Study on Expression Characteristic of alpha B-crystallin in Skeletal Muscle Cell after One Time High-intensity Eccentric Exercise

Objective: The purpose of this study is to examine the characteristic expression of alpha B-crystallin in skeletal muscle cell after rats did one time high-intensity eccentric exercise. The paper investigated the mechanism protective effects of alpha B-crystallin in skeletal muscle. Methods: 24 adult male Wistar rats were divided into control group (n=8), immediately after exercise group (n=8), 24h after exercise group (n=8). Exercise groups did one time intensity eccentric exercise. After eccentric exercise, rats gastrocnemius muscle was take to cryo-section. Then the research used immunofluorence methods to examine alpha B-crystallin expression characteristic in muscle cell. Result: In control group, alpha B-crystallin expression is low in muscle cell; In immediately after exercise group and 24h after exercise group, alpha B-crystallin expression of muscle cell is very high. One remarkable characteristic is that alpha B-crystallin expression signal is near muscle cell membrane and Z line. Conclusion: After