Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy
C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spina...
Gespeichert in:
| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2012-09, Vol.48 (72), p.9059-9061 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 9061 |
|---|---|
| container_issue | 72 |
| container_start_page | 9059 |
| container_title | Chemical communications (Cambridge, England) |
| container_volume | 48 |
| creator | Nakayama, Shizuka Luo, Yiling Zhou, Jie Dayie, T Kwaku Sintim, Herman O |
| description | C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module. |
| doi_str_mv | 10.1039/c2cc34379g |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1033534882</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1033534882</sourcerecordid><originalsourceid>FETCH-LOGICAL-p141t-e4689c382a8006fdd467700f7dadf4f5c8603fc950d718d3d410c4338e33a0303</originalsourceid><addsrcrecordid>eNo1kEtLxDAcxIMg7rp68QNIjl6iSf9Jmx5l0VWoj4OCtxLzKJW2qXkc9ttbcZ3LXGaGH4PQBaPXjEJ9owutgUNVd0dozaDkRHD5sUKnMX7RRUzIE7QqCil4xeQaNc9q8qMfVMBuyD7YqO2UsLHJ6tT7CXuHNTE92T294hz7qcMKj97k34aakxptwDEFlWy3P0PHTg3Rnh98g97v7962D6R52T1ubxsyM84SsbyUtQZZKElp6YzhZVVR6iqjjONOaFlScLoW1CyMBgxnVHMAaQEUBQobdPW3Owf_nW1M7dgv3MOgJutzbJcnQACXsliil4do_hytaefQjyrs2_8H4AfrMFow</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1033534882</pqid></control><display><type>article</type><title>Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Nakayama, Shizuka ; Luo, Yiling ; Zhou, Jie ; Dayie, T Kwaku ; Sintim, Herman O</creator><creatorcontrib>Nakayama, Shizuka ; Luo, Yiling ; Zhou, Jie ; Dayie, T Kwaku ; Sintim, Herman O</creatorcontrib><description>C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.</description><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/c2cc34379g</identifier><identifier>PMID: 22854718</identifier><language>eng</language><publisher>England</publisher><subject>Aptamers, Nucleotide - chemistry ; Aptamers, Nucleotide - genetics ; Base Sequence ; Biosensing Techniques - methods ; Cyclic GMP - analogs & derivatives ; Cyclic GMP - analysis ; Riboswitch ; RNA, Plant - chemistry ; Spectrometry, Fluorescence</subject><ispartof>Chemical communications (Cambridge, England), 2012-09, Vol.48 (72), p.9059-9061</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22854718$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakayama, Shizuka</creatorcontrib><creatorcontrib>Luo, Yiling</creatorcontrib><creatorcontrib>Zhou, Jie</creatorcontrib><creatorcontrib>Dayie, T Kwaku</creatorcontrib><creatorcontrib>Sintim, Herman O</creatorcontrib><title>Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.</description><subject>Aptamers, Nucleotide - chemistry</subject><subject>Aptamers, Nucleotide - genetics</subject><subject>Base Sequence</subject><subject>Biosensing Techniques - methods</subject><subject>Cyclic GMP - analogs & derivatives</subject><subject>Cyclic GMP - analysis</subject><subject>Riboswitch</subject><subject>RNA, Plant - chemistry</subject><subject>Spectrometry, Fluorescence</subject><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kEtLxDAcxIMg7rp68QNIjl6iSf9Jmx5l0VWoj4OCtxLzKJW2qXkc9ttbcZ3LXGaGH4PQBaPXjEJ9owutgUNVd0dozaDkRHD5sUKnMX7RRUzIE7QqCil4xeQaNc9q8qMfVMBuyD7YqO2UsLHJ6tT7CXuHNTE92T294hz7qcMKj97k34aakxptwDEFlWy3P0PHTg3Rnh98g97v7962D6R52T1ubxsyM84SsbyUtQZZKElp6YzhZVVR6iqjjONOaFlScLoW1CyMBgxnVHMAaQEUBQobdPW3Owf_nW1M7dgv3MOgJutzbJcnQACXsliil4do_hytaefQjyrs2_8H4AfrMFow</recordid><startdate>20120918</startdate><enddate>20120918</enddate><creator>Nakayama, Shizuka</creator><creator>Luo, Yiling</creator><creator>Zhou, Jie</creator><creator>Dayie, T Kwaku</creator><creator>Sintim, Herman O</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20120918</creationdate><title>Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy</title><author>Nakayama, Shizuka ; Luo, Yiling ; Zhou, Jie ; Dayie, T Kwaku ; Sintim, Herman O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p141t-e4689c382a8006fdd467700f7dadf4f5c8603fc950d718d3d410c4338e33a0303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aptamers, Nucleotide - chemistry</topic><topic>Aptamers, Nucleotide - genetics</topic><topic>Base Sequence</topic><topic>Biosensing Techniques - methods</topic><topic>Cyclic GMP - analogs & derivatives</topic><topic>Cyclic GMP - analysis</topic><topic>Riboswitch</topic><topic>RNA, Plant - chemistry</topic><topic>Spectrometry, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakayama, Shizuka</creatorcontrib><creatorcontrib>Luo, Yiling</creatorcontrib><creatorcontrib>Zhou, Jie</creatorcontrib><creatorcontrib>Dayie, T Kwaku</creatorcontrib><creatorcontrib>Sintim, Herman O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakayama, Shizuka</au><au>Luo, Yiling</au><au>Zhou, Jie</au><au>Dayie, T Kwaku</au><au>Sintim, Herman O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2012-09-18</date><risdate>2012</risdate><volume>48</volume><issue>72</issue><spage>9059</spage><epage>9061</epage><pages>9059-9061</pages><eissn>1364-548X</eissn><abstract>C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.</abstract><cop>England</cop><pmid>22854718</pmid><doi>10.1039/c2cc34379g</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1364-548X |
| ispartof | Chemical communications (Cambridge, England), 2012-09, Vol.48 (72), p.9059-9061 |
| issn | 1364-548X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1033534882 |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Aptamers, Nucleotide - chemistry Aptamers, Nucleotide - genetics Base Sequence Biosensing Techniques - methods Cyclic GMP - analogs & derivatives Cyclic GMP - analysis Riboswitch RNA, Plant - chemistry Spectrometry, Fluorescence |
| title | Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T04%3A53%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nanomolar%20fluorescent%20detection%20of%20c-di-GMP%20using%20a%20modular%20aptamer%20strategy&rft.jtitle=Chemical%20communications%20(Cambridge,%20England)&rft.au=Nakayama,%20Shizuka&rft.date=2012-09-18&rft.volume=48&rft.issue=72&rft.spage=9059&rft.epage=9061&rft.pages=9059-9061&rft.eissn=1364-548X&rft_id=info:doi/10.1039/c2cc34379g&rft_dat=%3Cproquest_pubme%3E1033534882%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1033534882&rft_id=info:pmid/22854718&rfr_iscdi=true |