Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy

Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy

C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spina...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2012-09, Vol.48 (72), p.9059-9061
Hauptverfasser: Nakayama, Shizuka, Luo, Yiling, Zhou, Jie, Dayie, T Kwaku, Sintim, Herman O
Format: Artikel
Sprache:eng
Schlagworte:
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - genetics
Base Sequence
Biosensing Techniques - methods
Cyclic GMP - analogs & derivatives
Cyclic GMP - analysis
Riboswitch
RNA, Plant - chemistry
Spectrometry, Fluorescence
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Zusammenfassung:C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.
ISSN:1364-548X
DOI:10.1039/c2cc34379g