Optimized conditions for primary culture of pituitary cells from the Atlantic cod ( Gadus morhua ). The importance of osmolality, pCO2 , and pH

Optimized conditions for primary culture of pituitary cells from the Atlantic cod ( Gadus morhua ). The importance of osmolality, pCO2 , and pH

Highlights ► Adapting mammalian primary cell culture protocols to teleosts (Atlantic cod). ► Adjusting medium osmolality, pCO2 and pH increase viability and functionality. ► Physiological osmolality in all solutions increases V m and survival time. ► Physiological pCO2 and pH stabilize V m and furth...

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Veröffentlicht in:General and comparative endocrinology 2012-09, Vol.178 (2), p.206-215
Hauptverfasser: Hodne, Kjetil, von Krogh, Kristine, Weltzien, Finn-Arne, Sand, Olav, Haug, Trude M
Format: Artikel
Sprache:eng
Schlagworte:
action potentials
Animals
aquatic habitat
blood serum
body temperature
buffering capacity
calves
Carbon Dioxide
Cell Culture Techniques - methods
cell viability
Cells, Cultured
cod (fish)
culture media
Culture protocol
direct contact
Electrophysiology
endocrinology
Endocrinology & Metabolism
Gadus morhua
genes
gills
Hydrogen-Ion Concentration
Membrane potential
microsatellite repeats
neonates
osmolality
Osmolar Concentration
Pituitary Gland - cytology
qPCR
spawning
Teleost
Viability
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Zusammenfassung:Highlights ► Adapting mammalian primary cell culture protocols to teleosts (Atlantic cod). ► Adjusting medium osmolality, pCO2 and pH increase viability and functionality. ► Physiological osmolality in all solutions increases V m and survival time. ► Physiological pCO2 and pH stabilize V m and further prolong survival time. ► Serum supplement is not crucial, but increase viability and affect gene expression.
ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2012.06.005