Production of recombinant orange-spotted grouper ( Epinephelus coioides ) follicle-stimulating hormone (FSH) in single-chain form and dimer form by Pichia pastoris and their biological activities

Production of recombinant orange-spotted grouper ( Epinephelus coioides ) follicle-stimulating hormone (FSH) in single-chain form and dimer form by Pichia pastoris and their biological activities

Highlights ► Single-chain form and dimer form of grouper FSH were successfully produced by Pichia pastoris . ► Recombinant FSHs had similar functions, though dimer form of FSH functioned weaker in in vitro . ► Recombinant FSH could activate grouper FSH receptor. ► Recombinant FSH stimulated in vitro...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:General and comparative endocrinology 2012-09, Vol.178 (2), p.237-249
Hauptverfasser: Chen, Jun, Zhang, Yanhong, Tang, Zhiguo, Mao, Jiewei, Kuang, Zhonglei, Qin, Chaobin, Li, Wensheng
Format: Artikel
Sprache:eng
Schlagworte:
adults
animal ovaries
Animals
Bass - genetics
Bass - metabolism
bioactive properties
blood serum
Blotting, Western
Early ovarian development
Endocrinology & Metabolism
Epinephelus coioides
Female
Follicle Stimulating Hormone - biosynthesis
Follicle Stimulating Hormone - chemistry
Follicle Stimulating Hormone - genetics
Follicle-stimulating hormone
follicle-stimulating hormone receptors
gene expression
genes
glycosylation
grouper
intraperitoneal injection
Male
mass spectrometry
messenger RNA
ovarian development
Pichia - genetics
Pichia - metabolism
Pichia pastoris
polypeptides
recombinant proteins
secretion
Steroidogenesis
testosterone
Western blotting
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Highlights ► Single-chain form and dimer form of grouper FSH were successfully produced by Pichia pastoris . ► Recombinant FSHs had similar functions, though dimer form of FSH functioned weaker in in vitro . ► Recombinant FSH could activate grouper FSH receptor. ► Recombinant FSH stimulated in vitro and in vivo sex steroid hormones release. ► Recombinant FSH induced early ovarian development in juvenile grouper.
ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2012.05.009