Oleaginous extract from the fruits Pterodon pubescens Benth induces antinociception in animal models of acute and chronic pain

Pterodon pubescens Benth is a medicinal plant commonly used for therapeutic purposes in folk medicine for rheumatic diseases' treatment. In the present work we analyzed the chemical composition of the oleaginous extract of P. pubescens Benth (OEPp) and extended the antinociceptive effect of OEPp evaluating its role on animal models of acute and chronic pain. The antinociceptive and antiedematogenic effects of OEPp (3–100mg/kg, i.g.) were evaluated in the formalin test; mechanical allodynia in the postoperative pain and complex regional pain syndrome type-I (CRPS-I) animal models; and thermal hyperalgesia was induced by plantar incision. Finally, we performed a phytochemical analysis of OEPp. The chemical composition of OEPp was analyzed by mass spectrometry (GC/MS) and eight sesquiterpene compounds were identified, i.e. three major sesquiterpene (E-cariofilene, γ-muurolene, biciclogermacrene), and nine vouacapane diterpenes, four of which showed in major concentration (6α-acetoxyvouacapane, 6α,7β-dimetoxivouacapan-17-ene, 6α-acetoxy,7β-hidroxyvouacapane, 6α,7β-diacetoxycouacapane). Furthermore, the results of the present study demonstrate, for the first time, that the OEPp reduced mechanical allodynia in the postoperative pain and CRPS-I animal models. OEPp also increased the paw withdrawal latency in hot- and cold-plate tests in the postoperative pain model. In addition, the present work confirms and extends previous data from literature showing that systemic administration of OEPp caused significant inhibition against both phases of pain response to formalin intraplantar injection and edema formation. Together, present and previous findings show that OEPp given intra-gastrically caused significant inhibition against both phases of formalin intraplantar injection and effectively inhibited mechanical and thermal hyperalgesia in the postoperative pain and CRPS-I animal models. P. pubescens, popularly known in Brazil as ‘sucupira branca’, has been widely used by domestic medicine as an anti-inflammatory, the present study demonstrate, for the first time that the OEPp reduced mechanical allodynia in the postoperative pain and Complex Regional Syndorme Type I animal models. In addition, the present work confirms and extends previous data from literature showing that systemic administration of OEPp caused significant inhibition of inflammatory pain assessed in the formalin test. [Display omitted]