A glucose oxidase immobilization platform for glucose biosensor using ZnO hollow nanospheres
A good route (template-directed synthetic route) for the fabrication of ZnO hollow nanospheres (ZnO-HNSPs) was proposed. ZnO hollow nanosphere is a wonderful platform to immobilize glucose oxidase for glucose biosensor owing to the high specific surface area and high isoelectric point (IEP). Along w...
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| Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2011-07, Vol.155 (1), p.304-310 |
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| creator | Fang, Bin Zhang, Cuihong Wang, Guangfeng Wang, Meifang Ji, Yulan |
| description | A good route (template-directed synthetic route) for the fabrication of ZnO hollow nanospheres (ZnO-HNSPs) was proposed. ZnO hollow nanosphere is a wonderful platform to immobilize glucose oxidase for glucose biosensor owing to the high specific surface area and high isoelectric point (IEP). Along with nafion and glucose oxidase (GOD), a glucose sensor was designed. Nafion/ZnO-HNSPs/GOD/GCE displays higher catalytic activity toward the glucose oxidation than Nafion/ZnO nano-Flowers/GOD/GCE. Linear response was obtained over a concentration range from 5.0
×
10
−3
mM to 13.15
mM with a detection limit of 1.0
μM (S/N
=
3), and the sensitivity was 65.82
μA/(mM
cm
2). Satisfyingly, the Nafion/ZnO-HNSPs/GOD/GCE could effectively avoid the interferences from the common interfering species such as uric acid (UA), ascorbic acid (AA), dopamine (DA) and fructose. The Nafion/ZnO-HNSPs/GOD modified electrode allows high sensitivity, excellently selective, stable, and fast amperometric sensing of glucose and thus is promising for the future development of glucose sensors. |
| doi_str_mv | 10.1016/j.snb.2010.12.040 |
| format | Article |
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×
10
−3
mM to 13.15
mM with a detection limit of 1.0
μM (S/N
=
3), and the sensitivity was 65.82
μA/(mM
cm
2). Satisfyingly, the Nafion/ZnO-HNSPs/GOD/GCE could effectively avoid the interferences from the common interfering species such as uric acid (UA), ascorbic acid (AA), dopamine (DA) and fructose. The Nafion/ZnO-HNSPs/GOD modified electrode allows high sensitivity, excellently selective, stable, and fast amperometric sensing of glucose and thus is promising for the future development of glucose sensors.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2010.12.040</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Ascorbic acid ; biosensors ; catalytic activity ; detection limit ; dopamine ; electrodes ; fructose ; Glucose ; Glucose oxidase ; Hollow nanosphere ; isoelectric point ; Modified electrode ; nanospheres ; oxidation ; surface area ; uric acid ; zinc oxide ; ZnO</subject><ispartof>Sensors and actuators. B, Chemical, 2011-07, Vol.155 (1), p.304-310</ispartof><rights>2010 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-8811086ed169b8ea27bb0aec15fda53b3ff2c85cedab772b32fdd056d87c13c63</citedby><cites>FETCH-LOGICAL-c420t-8811086ed169b8ea27bb0aec15fda53b3ff2c85cedab772b32fdd056d87c13c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.snb.2010.12.040$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Fang, Bin</creatorcontrib><creatorcontrib>Zhang, Cuihong</creatorcontrib><creatorcontrib>Wang, Guangfeng</creatorcontrib><creatorcontrib>Wang, Meifang</creatorcontrib><creatorcontrib>Ji, Yulan</creatorcontrib><title>A glucose oxidase immobilization platform for glucose biosensor using ZnO hollow nanospheres</title><title>Sensors and actuators. B, Chemical</title><description>A good route (template-directed synthetic route) for the fabrication of ZnO hollow nanospheres (ZnO-HNSPs) was proposed. ZnO hollow nanosphere is a wonderful platform to immobilize glucose oxidase for glucose biosensor owing to the high specific surface area and high isoelectric point (IEP). Along with nafion and glucose oxidase (GOD), a glucose sensor was designed. Nafion/ZnO-HNSPs/GOD/GCE displays higher catalytic activity toward the glucose oxidation than Nafion/ZnO nano-Flowers/GOD/GCE. Linear response was obtained over a concentration range from 5.0
×
10
−3
mM to 13.15
mM with a detection limit of 1.0
μM (S/N
=
3), and the sensitivity was 65.82
μA/(mM
cm
2). Satisfyingly, the Nafion/ZnO-HNSPs/GOD/GCE could effectively avoid the interferences from the common interfering species such as uric acid (UA), ascorbic acid (AA), dopamine (DA) and fructose. The Nafion/ZnO-HNSPs/GOD modified electrode allows high sensitivity, excellently selective, stable, and fast amperometric sensing of glucose and thus is promising for the future development of glucose sensors.</description><subject>Ascorbic acid</subject><subject>biosensors</subject><subject>catalytic activity</subject><subject>detection limit</subject><subject>dopamine</subject><subject>electrodes</subject><subject>fructose</subject><subject>Glucose</subject><subject>Glucose oxidase</subject><subject>Hollow nanosphere</subject><subject>isoelectric point</subject><subject>Modified electrode</subject><subject>nanospheres</subject><subject>oxidation</subject><subject>surface area</subject><subject>uric acid</subject><subject>zinc oxide</subject><subject>ZnO</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMoWKs_wJN79LLrJNndpHgq4hcUetBeRAhJNtum7CY12fr1602pePTyDjM88zLzInSOocCA66t1EZ0qCOx6UkAJB2iEOaM5BcYO0QgmpMpLgOoYncS4BoCS1jBCr9Ns2W21jybzn7aRqdq-98p29lsO1rts08mh9aHPkvyxyiZ1MU220bpl9uLm2cp3nf_InHQ-blYmmHiKjlrZRXP2W8docXf7fPOQz-b3jzfTWa5LAkPOOcbAa9PgeqK4kYQpBdJoXLWNrKiibUs0r7RppGKMKErapoGqbjjTmOqajtHl3ncT_NvWxEH0NmrTddIZv40CA-HAOKvLhOI9qoOPMZhWbILtZfhKkNglKdYiJSl2SQpMREoy7Vzsd1rphVwGG8XiKQEVAMaMUZaI6z1h0pfv1gQRtTUuXWyD0YNovP3H_wftNoca</recordid><startdate>20110705</startdate><enddate>20110705</enddate><creator>Fang, Bin</creator><creator>Zhang, Cuihong</creator><creator>Wang, Guangfeng</creator><creator>Wang, Meifang</creator><creator>Ji, Yulan</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QR</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110705</creationdate><title>A glucose oxidase immobilization platform for glucose biosensor using ZnO hollow nanospheres</title><author>Fang, Bin ; Zhang, Cuihong ; Wang, Guangfeng ; Wang, Meifang ; Ji, Yulan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-8811086ed169b8ea27bb0aec15fda53b3ff2c85cedab772b32fdd056d87c13c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Ascorbic acid</topic><topic>biosensors</topic><topic>catalytic activity</topic><topic>detection limit</topic><topic>dopamine</topic><topic>electrodes</topic><topic>fructose</topic><topic>Glucose</topic><topic>Glucose oxidase</topic><topic>Hollow nanosphere</topic><topic>isoelectric point</topic><topic>Modified electrode</topic><topic>nanospheres</topic><topic>oxidation</topic><topic>surface area</topic><topic>uric acid</topic><topic>zinc oxide</topic><topic>ZnO</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fang, Bin</creatorcontrib><creatorcontrib>Zhang, Cuihong</creatorcontrib><creatorcontrib>Wang, Guangfeng</creatorcontrib><creatorcontrib>Wang, Meifang</creatorcontrib><creatorcontrib>Ji, Yulan</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fang, Bin</au><au>Zhang, Cuihong</au><au>Wang, Guangfeng</au><au>Wang, Meifang</au><au>Ji, Yulan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A glucose oxidase immobilization platform for glucose biosensor using ZnO hollow nanospheres</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2011-07-05</date><risdate>2011</risdate><volume>155</volume><issue>1</issue><spage>304</spage><epage>310</epage><pages>304-310</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>A good route (template-directed synthetic route) for the fabrication of ZnO hollow nanospheres (ZnO-HNSPs) was proposed. ZnO hollow nanosphere is a wonderful platform to immobilize glucose oxidase for glucose biosensor owing to the high specific surface area and high isoelectric point (IEP). Along with nafion and glucose oxidase (GOD), a glucose sensor was designed. Nafion/ZnO-HNSPs/GOD/GCE displays higher catalytic activity toward the glucose oxidation than Nafion/ZnO nano-Flowers/GOD/GCE. Linear response was obtained over a concentration range from 5.0
×
10
−3
mM to 13.15
mM with a detection limit of 1.0
μM (S/N
=
3), and the sensitivity was 65.82
μA/(mM
cm
2). Satisfyingly, the Nafion/ZnO-HNSPs/GOD/GCE could effectively avoid the interferences from the common interfering species such as uric acid (UA), ascorbic acid (AA), dopamine (DA) and fructose. The Nafion/ZnO-HNSPs/GOD modified electrode allows high sensitivity, excellently selective, stable, and fast amperometric sensing of glucose and thus is promising for the future development of glucose sensors.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2010.12.040</doi><tpages>7</tpages></addata></record> |
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| ispartof | Sensors and actuators. B, Chemical, 2011-07, Vol.155 (1), p.304-310 |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | Ascorbic acid biosensors catalytic activity detection limit dopamine electrodes fructose Glucose Glucose oxidase Hollow nanosphere isoelectric point Modified electrode nanospheres oxidation surface area uric acid zinc oxide ZnO |
| title | A glucose oxidase immobilization platform for glucose biosensor using ZnO hollow nanospheres |
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