Verrucosispora wenchangensis sp. nov., isolated from mangrove soil

An actinomycete strain 234402 T was isolated from a mangrove soil sample collected in Wenchang, China. Phylogenetic analysis of the 16S rRNA gene sequence of strain 234402 T indicated that the highest similarity was to Verrucosispora sediminis MS426 T (99.25%). The cell wall contained meso -diaminopimelic acid. The major menaquinones were MK-9(H 4 ) and MK-9(H 6 ), with MK-9(H 8 ) as minor components. The characteristic whole-cell sugars were xylose, mannose and glucose. The phospholipid profile was found to comprise phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside and an unknown phospholipid. The DNA G+C content was 69.2 mol%. The results of physiological and biochemical tests and low DNA–DNA relatedness demonstrated strain 234402 T could be readily distinguished from the closely related Verrucosispora species. On the basis of these phenotypic and genotypic data, strain 234402 T represents a novel species of the genus Verrucosispora , for which the name Verrucosispora wenchangensis sp. nov. is proposed. The type strain is 234402 T (=CCTCC AA 2011018 T =DSM 45674 T ).