Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine

Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine

We report the first site-specific genetic encoding of photocaged tyrosine into proteins in mammalian cells. By photocaging Tyr701 of STAT1 we demonstrate that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells.

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Bibliographische Detailangaben
Veröffentlicht in:Journal of the American Chemical Society 2012-07, Vol.134 (29), p.11912-11915
Hauptverfasser: Arbely, Eyal, Torres-Kolbus, Jessica, Deiters, Alexander, Chin, Jason W
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acyl-tRNA Synthetases - genetics
Escherichia coli - genetics
Escherichia coli - metabolism
HEK293 Cells
Humans
Light
Methanosarcina - enzymology
Methanosarcina - genetics
Mutation
Phosphorylation
Protein Engineering - methods
Signal Transduction
STAT1 Transcription Factor - genetics
STAT1 Transcription Factor - metabolism
Tyrosine - genetics
Tyrosine - metabolism
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Beschreibung
Zusammenfassung:We report the first site-specific genetic encoding of photocaged tyrosine into proteins in mammalian cells. By photocaging Tyr701 of STAT1 we demonstrate that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja3046958