Studies of Mg2+/Ca2+ complexes of naturally occurring dinucleotides: potentiometric titrations, NMR, and molecular dynamics
Dinucleotides (Np n N′; N and N′ are A, U, G, or C, n = 2–7) are naturally occurring physiologically active compounds. Despite the interest in dinucleotides, the composition of their complexes with metal ions as well as their conformations and species distribution in living systems are understudied...
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Veröffentlicht in: | Journal of biological inorganic chemistry 2012-08, Vol.17 (6), p.861-879 |
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Sprache: | eng |
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Zusammenfassung: | Dinucleotides (Np
n
N′; N and N′ are A, U, G, or C,
n
= 2–7) are naturally occurring physiologically active compounds. Despite the interest in dinucleotides, the composition of their complexes with metal ions as well as their conformations and species distribution in living systems are understudied. Therefore, we investigated a series of Mg
2+
and Ca
2+
complexes of Np
n
N′s. Potentiometric titrations indicated that a longer dinucleotide polyphosphate (N is A or G,
n
= 3–5) linker yields more stable complexes (e.g., log
K
of 2.70, 3.27, and 3.73 for Ap
n
A–Mg
2+
,
n
= 3, 4, 5, respectively). The base (A or G) or ion (Mg
2+
or Ca
2+
) has a minor effect on
values. In a physiological medium, the longer Ap
n
As (
n
= 4, 5) are predicted to occur mostly as the Mg
2+
/Ca
2+
complexes.
31
P NMR monitored titrations of Np
n
N′s with Mg
2+
/Ca
2+
ions showed that the middle phosphates of the dinucleotides coordinate with Mg
2+
/Ca
2+
. Multidimensional potential of mean force (PMF) molecular dynamics (MD) simulations suggest that Ap
2
A and Ap
4
A coordinate Mg
2+
and Ca
2+
ions in both inner-sphere and outer-sphere modes. The PMF MD simulations additionally provide a detailed picture of the possible coordination sites, as well as the cation binding process. Moreover, both NMR and MD simulations showed that the conformation of the nucleoside moieties in Np
n
N′–Mg
2+
/Ca
2+
complexes remains the same as that of free mononucleotides.
Graphical abstract
Dinucleotides form complexes with Mg
2+
/Ca
2+
ions which coordinate to the middle phosphate groups in the polyphosphate chain. Mg
2+
/Ca
2+
coordination slightly increases intramolecular
π
-stacking interactions in the complexes, whereas the conformation of nucleoside moieties remains almost the same as in the corresponding Na
+
salts. Considering physiological Mg
2+
/Ca
2+
concentrations, the Ap
4
A–Mg
2+
/Ap
5
A–Mg
2+
and Ap
4
A–Ca
2+
/Ap
5
A–Ca
2+
species are expected to be dominant (approximately 70–90 %) in living systems versus the free dinucleotide. |
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ISSN: | 0949-8257 1432-1327 |
DOI: | 10.1007/s00775-012-0903-2 |