Comparative Quality Assessment of Buffalo (Bubalus bubalis) Semen Chilled (5°C) in Egg Yolk‐ and Soya Milk–Based Extenders
Egg yolk–Tris is most commonly used semen extender; however, its use involves hygienic risk, interference with fertility and poor microscopic examination. Therefore, replacement of egg yolk with a plant‐based component with protective effects on spermatozoa would be advantageous. In present study, w...
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Veröffentlicht in: | Reproduction in domestic animals 2012-08, Vol.47 (4), p.596-600 |
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Sprache: | eng |
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Zusammenfassung: | Egg yolk–Tris is most commonly used semen extender; however, its use involves hygienic risk, interference with fertility and poor microscopic examination. Therefore, replacement of egg yolk with a plant‐based component with protective effects on spermatozoa would be advantageous. In present study, we observed effect of soya milk–based extenders on dilution and liquid preservation of Murrah buffalo bull semen at 5°C up to 72 h in comparison with conventional egg yolk–Tris extender (Ext.1). In experiment one, a total of 32 buffalo semen ejaculates from four animals were extended and preserved at 5°C for 72 h in soya milk–based extender (Ext.2) with different percentages (10%, 15%, 20%, 25% and 30%) of soya milk for optimization of soya milk concentration. Semen quality was assessed for individual motility, viability, membrane integrity and acrosome integrity at 0, 24, 48 and 72 h of liquid preservation. The results of experiment one indicated that 25% soya milk is an optimum concentration for buffalo bull semen extender preparation. A modified method was used to prepare another soya milk–based extender (Ext.3). In the second experiment, two soya extenders (Ext.2 and 3) with optimized concentration (25%) of soya milk were comparatively assessed with egg yolk–Tris extender (Ext.1) for semen quality parameters at 0, 24, 48 and 72 h of liquid preservation. The individual sperm motility at 0 and 24 h following dilution were found non‐significant among extenders. However, after 48 h of dilution, individual motility in Ext.3 was observed significantly (p |
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ISSN: | 0936-6768 1439-0531 |
DOI: | 10.1111/j.1439-0531.2011.01928.x |