Effect of sequential medium on in vitro culture of goat ovarian cortical tissue

Effect of sequential medium on in vitro culture of goat ovarian cortical tissue

A sequential medium was evaluated on the survival, activation and growth rates of caprine preantral follicles submitted to a long-term culture period, aiming to establish an ideal in vitro culture system. Ovarian fragments were cultured for 16 days in α-MEM+ alone or supplemented with hormones (GH a...

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Veröffentlicht in:Animal reproduction science 2012-06, Vol.132 (3-4), p.159-168
Hauptverfasser: Magalhães-Padilha, D.M, Andrade, P.M, Sales, E.T, Araujo, V.R, Lima, I.M.T, Castro, S.V, Faustino, L.R, Lopes, C.A.P, Campello, C.C, Báo, S.N, Gastal, M.O, Gastal, E.L, Figueiredo, J.R
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Caprine
Culture Media
Female
Follicle Stimulating Hormone - pharmacology
follicle-stimulating hormone
Folliculogenesis
FSH
goats
Goats - physiology
Growth Hormone - pharmacology
in vitro culture
Ovary - drug effects
Ovary - physiology
Ovary - ultrastructure
Preantral follicle
Time Factors
Tissue Culture Techniques - veterinary
ultrastructure
viability
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Zusammenfassung:A sequential medium was evaluated on the survival, activation and growth rates of caprine preantral follicles submitted to a long-term culture period, aiming to establish an ideal in vitro culture system. Ovarian fragments were cultured for 16 days in α-MEM+ alone or supplemented with hormones (GH and/or FSH) added sequentially on different days of culture. Ovarian fragments were cultured in the first (days 0–8) and second (days 8–16) halves of the culture period, generating 10 treatments: α-MEM+/α-MEM+, FSH/FSH, FSH/GH, FSH/FSH+GH, GH/GH, GH/FSH, GH/FSH+GH, FSH+GH/FSH+GH, FSH+GH/FSH and FSH+GH/GH. Follicle morphology, viability and ultrastructure were analyzed. After day 1 of culture, FSH treatments maintained the percentage of normal follicles similar to the fresh control. At day 16 of culture, the treatment FSH/GH showed the highest (P
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2012.05.017