In vitro evaluation of ram sperm frozen with glycerol, ethylene glycol or acetamide

The aim was to assess the in vitro effect of glycerol, ethylene glycol or acetamide on frozen-thawed ram spermatozoa. Aliquots of each sixteen ejaculates from four rams of the Morada Nova breed were diluted in Tris-egg yolk with glycerol (5%), ethylene glycol (3% or 5%) or acetamide (3% or 5%) and f...

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Veröffentlicht in:	Animal reproduction science 2012-06, Vol.132 (3-4), p.155-158
Hauptverfasser:	Silva, E.C.B, Cajueiro, J.F.P, Silva, S.V, Vidal, A.H, Soares, P.C, Guerra, M.M.P
Format:	Artikel
Sprache:	eng
Schlagworte:	acetamide Acetamides - chemistry Acetamides - pharmacology acrosome Animals cryopreservation Cryopreservation - methods Cryopreservation - veterinary Cryoprotectant cryoprotectants Cryoprotective Agents - chemistry Cryoprotective Agents - pharmacology ethylene glycol Ethylene Glycol - chemistry Ethylene Glycol - pharmacology Freezing glycerol Glycerol - chemistry Glycerol - pharmacology Male oxidative stress plasma membrane rams Semen Semen Preservation - methods Semen Preservation - veterinary Sheep Sheep - physiology sperm motility Spermatozoa - physiology thawing
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Zusammenfassung:	The aim was to assess the in vitro effect of glycerol, ethylene glycol or acetamide on frozen-thawed ram spermatozoa. Aliquots of each sixteen ejaculates from four rams of the Morada Nova breed were diluted in Tris-egg yolk with glycerol (5%), ethylene glycol (3% or 5%) or acetamide (3% or 5%) and frozen at −196°C. After thawing, progressive sperm motility was greater (P
ISSN:	0378-4320 1873-2232
DOI:	10.1016/j.anireprosci.2012.05.014