Mechanism of the Prokaryotic Transmembrane Disulfide Reduction Pathway and Its In Vitro Reconstitution from Purified Components

Making your (Dsb) connection: The redox pathway bringing reducing equivalents from bacterial cytoplasm, across the inner membrane, to the three reductive Dsb pathways in the otherwise oxidizing periplasm (see scheme; TR=thioredoxin reductase, Trx=thioredoxin) is reconstituted from purified components. Transfer of reducing equivalents across the membrane is demonstrated and underlying mechanistic details are revealed.