High-throughput screening for cellobiose dehydrogenases by Prussian Blue in situ formation

High-throughput screening for cellobiose dehydrogenases by Prussian Blue in situ formation

Extracellular fungal flavocytochrome cellobiose dehydrogenase (CDH) is a promising enzyme for both bioelectronics and lignocellulose bioconversion. A selective high‐throughput screening assay for CDH in the presence of various fungal oxidoreductases was developed. It is based on Prussian Blue (PB) i...

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Veröffentlicht in:Biotechnology journal 2012-07, Vol.7 (7), p.919-930
Hauptverfasser: Vasilchenko, Liliya G., Ludwig, Roland, Yershevich, Olga P., Haltrich, Dietmar, Rabinovich, Mikhail L.
Format: Artikel
Sprache:eng
Schlagworte:
Agaricales - enzymology
Ascomycota - enzymology
Carbohydrate Dehydrogenases - analysis
Carbohydrate Dehydrogenases - chemistry
Carbohydrate Dehydrogenases - metabolism
Cellobiose - metabolism
Cellobiose dehydrogenase
Culture Media
Fe3+ reduction
Ferricyanide
Ferricyanides - metabolism
Ferrocyanides - metabolism
Fungal Proteins - analysis
Fungal Proteins - chemistry
Fungal Proteins - metabolism
High-throughput assay
High-Throughput Screening Assays - methods
Hydrogen-Ion Concentration
Prussian Blue
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Zusammenfassung:Extracellular fungal flavocytochrome cellobiose dehydrogenase (CDH) is a promising enzyme for both bioelectronics and lignocellulose bioconversion. A selective high‐throughput screening assay for CDH in the presence of various fungal oxidoreductases was developed. It is based on Prussian Blue (PB) in situ formation in the presence of cellobiose (
ISSN:1860-6768
1860-7314
DOI:10.1002/biot.201100480