Sugar consumption and ethanol fermentation by transporter-overexpressed xylose-metabolizing Saccharomyces cerevisiae harboring a xyloseisomerase pathway

Four kinds of transporters, HXT1 and HXY7 from Saccharomyces cerevisiae, and GXF1 and GXS1 from Candida intermedia, were overexpressed in xylose-metabolizing S. cerevisiae harboring a xyloseisomerase-based pathway. Overexpression of transporter enhanced sugar consumption and ethanol production, and...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2012-08, Vol.114 (2), p.209-211
Hauptverfasser:	Tanino, Takanori, Ito, Tomonori, Ogino, Chiaki, Ohmura, Naoto, Ohshima, Takayuki, Kondo, Akihiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldose-Ketose Isomerases - metabolism Biological and medical sciences Biotechnology Candida - genetics Candida - metabolism Candida intermedia ethanol Ethanol - metabolism Ethanol fermentation Ethanol production Fermentation Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - metabolism glucose Glucose - metabolism Glucose Transport Proteins, Facilitative - genetics Glucose Transport Proteins, Facilitative - metabolism Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Monosaccharide Transport Proteins - genetics Monosaccharide Transport Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Sugar consumption Transporter transporters xylose Xylose - metabolism Xyloseisomerase pathway
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Beschreibung
Zusammenfassung:	Four kinds of transporters, HXT1 and HXY7 from Saccharomyces cerevisiae, and GXF1 and GXS1 from Candida intermedia, were overexpressed in xylose-metabolizing S. cerevisiae harboring a xyloseisomerase-based pathway. Overexpression of transporter enhanced sugar consumption and ethanol production, and GXF1 was efficient for ethanol fermentation from both glucose and xylose.
ISSN:	1389-1723 1347-4421
DOI:	10.1016/j.jbiosc.2012.03.004