Mild Extraction Technique for Flavonoids from Leaves of Nypa fruiticans and Chemical Analysis of Its Components

Eight flavonoid derivatives： rutin, quercetin-3-glucoside, quercetin, luteolin-7-glucoside, isorhmnetin-3-sulphate, kaempferol-3,7-diglucoside,＇luteolin and kaempferol have been extracted and characterized from Nipa palm. Mild extraction technique involving the use of HPLC-DAD-MS was used. The structures of the flavonoids were determined on the basis of mass spectroscopy. Separation of the crude extract by paper chromatography （PC） on forestall as solvent system gave one major yellowish brown spot which had Rf value of 3.9. The Rf value and maximum absorption from UV spectroscopy were the same as those of quercetin standard. The most prominent compound was quercetin followed by three others： kaempferol-3,7-diglucoside, luteolin-7-glucoside, and isorhmnetin-3 -sulphate.