Colourimetric solid-phase extraction coupled with fibre optic reflectance spectroscopy for determination of ascorbic acid in pharmaceutical formulations

A redox colourimetric solid‐phase extraction (C‐SPE) procedure for the determination of ascorbic acid (AA) in pharmaceutical formulations was proposed. Iron (III)‐2,2′‐dipyridyl (Fe(III)‐Bpy) reagent solution was used as a colouring reagent for AA and the immobilization of the redox product onto Amb...

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Veröffentlicht in:Drug testing and analysis 2012-06, Vol.4 (6), p.493-499
Hauptverfasser: Filik, Hayati, Aksu, Duygu, Giray, Derya, Apak, Reşat
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Sprache:eng
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Zusammenfassung:A redox colourimetric solid‐phase extraction (C‐SPE) procedure for the determination of ascorbic acid (AA) in pharmaceutical formulations was proposed. Iron (III)‐2,2′‐dipyridyl (Fe(III)‐Bpy) reagent solution was used as a colouring reagent for AA and the immobilization of the redox product onto Amberlite XAD‐16 resin was achieved. The analyte in the sample reacted with a solid sorbent loaded with the colourimetric reagent (Fe(III)‐Bpy) and then quantified directly on the sorbent surface by using a fibre optic reflectance spectrometer (FORS). The amount of AA was reflectometrically determined in a few seconds with a total sample workup and readout time of ∼10 min using only 10‐ml sample volumes. The limit of detection (LOD) and quantification (LOQ) values were 0.18 and 0.6 mg L−1, respectively, and the linear dynamic range for AA extended up to 8.8 mg L−1. The C‐SPE for different extractions (n = 5) gave a relative standard deviation (RSD) of 2.9% at 5.28 mg L−1 AA level. Copyright © 2011 John Wiley & Sons, Ltd. A redox colorimetric solid‐phase extraction (C‐SPE) procedure for the determination of ascorbic acid (AA) in pharmaceutical formulations was proposed. Iron (III)‐2,2′‐dipyridyl (Fe(III)‐Bpy) reagent solution was used as a coloring reagent for AA and the immobilisation of the redox product onto Amberlite XAD‐16 resin was achieved. The analyte in the sample reacted with a solid sorbent loaded with the colorimetric reagent (Fe(III)‐Bpy) and then quantified directly on the sorbent surface by using a fiber optic reflectance spectrometer (FORS).
ISSN:1942-7603
1942-7611
DOI:10.1002/dta.267