Defined Electrical Stimulation Emphasizing Excitability for the Development and Testing of Engineered Skeletal Muscle

Electrical stimulation is required for the maturation of skeletal muscle and as a way to nondestructively monitor muscle development. However, the wrong stimulation parameters can result in electrochemical damage that impairs muscle development/regeneration. The goal of the current study was to determine what aspect of an electrical impulse, specifically the pulse amplitude or pulse width, was detrimental to engineered muscle function and subsequently how engineered muscle responded to continuous electrical stimulation for 24 h. Acute stimulation at a pulse amplitude greater than six-times rheobase resulted in a 2.4-fold increase in the half-relaxation time (32.3±0.49 ms vs. 77.4±4.35 ms; p