CAPRINE ARTHRITIS ENCEPHALITIS VIRUS DETECTED BY PCR AND NESTED PCR IN GOAT SEMEN

Caprine arthritis encephalitis is a multisystemic infectious disease caused by a lentivirus and may affect animals of any age or sex. The major symptoms are leukoencephalomyelitis, chronic interstitial pneumonia, arthritis and hardening interstitial mastitis. It has been reported that the testicles...

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Veröffentlicht in:Arquivos do Instituto Biológico (São Paulo) 2011-01, Vol.78 (4), p.599-603
Hauptverfasser: Gregory, L, Lara, MCCSH, Hasegawa, MY, Castro, R S, Rodrigues, JNM, Araujo, J, Keller, L W, Silva, LKF, Durigon, EL
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Sprache:eng
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Zusammenfassung:Caprine arthritis encephalitis is a multisystemic infectious disease caused by a lentivirus and may affect animals of any age or sex. The major symptoms are leukoencephalomyelitis, chronic interstitial pneumonia, arthritis and hardening interstitial mastitis. It has been reported that the testicles of infected animals present infected semen and a gradual inflammatory process. For the detection of the agent in semen samples, PCR and nested PCR techniques are characterized as methods with high specificity and sensibility that provide fast results. The objective of this study was to detect the presence of pro-Ientiviral DNA in the semen of caprine males in Sao Paulo State, Brazil, by the PCR and nested PCR molecular techniques. Five (29.4%) of the 17 samples presented positivity for the detection of the virus, proving the presence of viral particles in the semen of male goats seropositive for caprine arthritis encephalitis in this study.Original Abstract: A Artrite Encefalite Caprina e uma enfermidade de carater multissistemico afetando animais de qualquer idade e sexo; causado por um Lentivirus caprino pertencente ao grupo de Lentivirus de Pequenos Ruminantes. Os sintomas conhecidos sao leucoencefalomielite, pneumonia intersticial cronica, artrite e mastite intersticial endurativa. Foi descrito que machos soropositivos apresentavam semen infectado pelo Lentivirus e processo inflamatorio gradual do testiculo. A deteccao em amostras de semen pelas tecnicas de PCR e nested-PCR sao caracterizadas como metodos de altas especificidade e sensibilidade provendo resultados rapidos. O objetivo do estudo foi detectar, por meio da tecnica molecular de PCR e nested-PCR, a presenca de DNA pro-viral do Lentivirus caprino em amostras de semen de reprodutores criados no Estado de Sao Paulo. Das 17 amostras, cinco (29,4%) apresentaram a deteccao do virus provando que ha a presenca de particulas virais no semen dos caprinos reprodutores soropositivos a Atrite Encefalite Caprina estudados.
ISSN:0020-3653