Overexpression of interleukin-6 and -8, cell growth inhibition and morphological changes in 2-hydroxyethyl methacrylate-treated human dental pulp mesenchymal stem cells

Trubiani O, Cataldi A, De Angelis F, D’Arcangelo C, Caputi S. Overexpression of interleukin‐6 and ‐8, cell growth inhibition and morphological changes in 2‐hydroxyethyl methacrylate‐treated human dental pulp mesenchymal stem cells. International Endodontic Journal, 45, 19–25, 2012. Aim To evaluate morphological features, cell growth and interleukin‐6 (IL‐6) and interleukin‐8 (IL‐8) secretion in expanded ex vivo human dental pulp mesenchymal stem cells (DP‐MSCs) after exposure to 2‐hydroxyethyl methacrylate (HEMA). Methodology Dental pulp mesenchymal stem cells were derived from the dental pulps of 10 young donors. After in vitro isolation, DP‐MSCs were treated with 3 and 5 mmol L−1 HEMA, and after 24, 48 and 72 h of incubation, their morphological features, cell growth, IL‐6 and IL‐8 secretion were analysed. Differences in the cell growth and in the interleukin secretion were analysed for statistical significance with two‐way anova tests and the Holm–Sidak method for multiple comparisons. Results Dental pulp mesenchymal stem cells revealed a decrease in cell growth with both treatments (P