Mycogenesis of gold nanoparticles using a phytopathogen Alternaria alternata
The development of an eco-friendly and reliable process for the synthesis of gold nanomaterials (AuNPs) using microorganisms is gaining importance in the field of nanotechnology. In the present study, AuNPs have been synthesized by bio-reduction of chloroauric acid (HAuCl 4 ) using the fungal cultur...
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Veröffentlicht in: | Bioprocess and biosystems engineering 2012-05, Vol.35 (4), p.637-643 |
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Sprache: | eng |
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Zusammenfassung: | The development of an eco-friendly and reliable process for the synthesis of gold nanomaterials (AuNPs) using microorganisms is gaining importance in the field of nanotechnology. In the present study, AuNPs have been synthesized by bio-reduction of chloroauric acid (HAuCl
4
) using the fungal culture filtrate (FCF) of
Alternaria alternata
. The synthesis of the AuNPs was monitored by UV–visible spectroscopy. The particles thereby obtained were characterized by UV, dynamic light scattering (DLS), X-ray diffraction (XRD), energy dispersive X-ray (EDX) analysis, Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and transmission electron microscopy (TEM). Energy-dispersive X-ray study revealed the presence of gold in the nanoparticles. Fourier transform infrared spectroscopy confirmed the presence of a protein shell outside the nanoparticles which in turn also support their stabilization. Treatment of the fungal culture filtrate with aqueous Au
+
ions produced AuNPs with an average particle size of 12 ± 5 nm. This proposed mechanistic principal might serve as a set of design rule for the synthesis of nanostructures with desired architecture and can be amenable for the large scale commercial production and technical applications. |
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ISSN: | 1615-7591 1615-7605 |
DOI: | 10.1007/s00449-011-0646-4 |