(IscS-IscU)2 Complex Structures Provide Insights into Fe2S2 Biogenesis and Transfer

(IscS-IscU)2 Complex Structures Provide Insights into Fe2S2 Biogenesis and Transfer

The center of attention: IscS cysteine desulfurases and IscU scaffolds are involved in biological iron–sulfur cluster assembly. The X‐ray structure of an anaerobically produced, mutated (Fe2S2‐(IscS‐IscUD35A))2 complex reveals a cluster coordinated by three IscU cysteines and the IscS active cystein...

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Veröffentlicht in:Angewandte Chemie International Edition 2012-05, Vol.51 (22), p.5439-5442
Hauptverfasser: Marinoni, Elodie N., de Oliveira, Jaim S., Nicolet, Yvain, Raulfs, Estella C., Amara, Patricia, Dean, Dennis R., Fontecilla-Camps, Juan C.
Format: Artikel
Sprache:eng
Schlagworte:
Archaeoglobus fulgidus - metabolism
biogenesis
Carbon-Sulfur Lyases - chemistry
Carbon-Sulfur Lyases - metabolism
computational chemistry
Crystallography, X-Ray
Cysteine
Iron - metabolism
iron-sulfur clusters
Iron-Sulfur Proteins - chemistry
Iron-Sulfur Proteins - metabolism
Models, Chemical
Models, Molecular
Oxygen - metabolism
Protein Binding
Protein Conformation
scaffold proteins
Sulfur - metabolism
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Zusammenfassung:The center of attention: IscS cysteine desulfurases and IscU scaffolds are involved in biological iron–sulfur cluster assembly. The X‐ray structure of an anaerobically produced, mutated (Fe2S2‐(IscS‐IscUD35A))2 complex reveals a cluster coordinated by three IscU cysteines and the IscS active cysteine (see picture). In air‐exposed crystals the cluster is oxidized to an Fe2S–S center; D35 is essential for complex dissociation.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201201708