Ginsenoside Rb1 inhibits the carotid neointimal hyperplasia induced by balloon injury in rats via suppressing the phenotype modulation of vascular smooth muscle cells

This study aims to investigate the effects of ginsenoside Rb1 on vascular intimal hyperplasia in rats and explore the mechanisms. The rat vascular neointimal hyperplasia model was made by rubbing the endothelia of carotid artery with a balloon and Rb1 (10 and 30mg/kg/day) was given the day after sur...

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Veröffentlicht in:	European journal of pharmacology 2012-06, Vol.685 (1-3), p.126-132
Hauptverfasser:	Zhang, Shu, Deng, Jiang, Gao, Yang, Yang, Dan-li, Gong, Qi-hai, Huang, Xie-nan
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Balloon-injury Carotid Artery Diseases - drug therapy Carotid Artery Diseases - pathology Catheterization Cell Proliferation - drug effects Disease Models, Animal Dose-Response Relationship, Drug Gene Expression Regulation - drug effects Ginsenoside Rb1 Ginsenosides - administration & dosage Ginsenosides - pharmacology Hyperplasia Male Mitogen-Activated Protein Kinase 1 - drug effects Mitogen-Activated Protein Kinase 1 - metabolism Mitogen-Activated Protein Kinase 3 - drug effects Mitogen-Activated Protein Kinase 3 - metabolism Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism Myocytes, Smooth Muscle - drug effects Myocytes, Smooth Muscle - metabolism Neointima - pathology Neointimal hyperplasia Phenotype Phenotype modulation Phosphorylation - drug effects Phosphorylation extracellular signal-regulated kinase 1/2 (pERK1/2) Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Smooth muscle α-actin
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Zusammenfassung:	This study aims to investigate the effects of ginsenoside Rb1 on vascular intimal hyperplasia in rats and explore the mechanisms. The rat vascular neointimal hyperplasia model was made by rubbing the endothelia of carotid artery with a balloon and Rb1 (10 and 30mg/kg/day) was given the day after surgery for 14 consecutive days. The neointimal hyperplasia level and the degree of vascular smooth muscle cells (VSMCs) proliferation were evaluated by histopathology and by calculating the proliferating cell nuclear antigen (PCNA) positive expression percentage; protein expressions of PCNA, phosphorylation extracellular signal-regulated kinase 1/2 (pERK1/2), smooth muscle α-actin (SM α-actin), and the mRNA expressions of proto-oncogene c-myc, SM α-actin, SM-emb (embryonic smooth muscle myosin heavy chain) and p38 MAPK were detected by immunohistochemistry and Real Time RT-PCR, respectively. Compared with the endothelia rubbing model group, Rb1 10 and 30mg/kg/day medication significantly ameliorated the neointimal hyperplasia (P
ISSN:	0014-2999 1879-0712
DOI:	10.1016/j.ejphar.2012.04.032