Nanobiocomposite platform based on polyaniline-iron oxide-carbon nanotubes for bacterial detection
The nanocomposite based on polyaniline (PANI)–iron oxide nanoparticles (nFe3O4) and multi walled carbon-nanotubes (CNT) has been fabricated onto indium tin oxide (ITO) coated glass plate via facile electrochemical synthesis of polyaniline in presence of nFe3O4 (~20nm) and CNT (20–80nm in diameter)....
Gespeichert in:
Veröffentlicht in: | Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2012-08, Vol.86, p.30-37 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 37 |
---|---|
container_issue | |
container_start_page | 30 |
container_title | Bioelectrochemistry (Amsterdam, Netherlands) |
container_volume | 86 |
creator | Singh, Renu Verma, Rachna Sumana, G. Srivastava, Avanish Kumar Sood, Seema Gupta, Rajinder K. Malhotra, B.D. |
description | The nanocomposite based on polyaniline (PANI)–iron oxide nanoparticles (nFe3O4) and multi walled carbon-nanotubes (CNT) has been fabricated onto indium tin oxide (ITO) coated glass plate via facile electrochemical synthesis of polyaniline in presence of nFe3O4 (~20nm) and CNT (20–80nm in diameter). The results of transmission electron microscopic studies show evidence of coating of PANI and nFe3O4 onto the CNT. The PANI-nFe3O4–CNT/ITO nanoelectrode has been characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy studies. The biotinylated nucleic acid probe sequence consisting of 20 bases has been immobilized onto PANI–nFe3O4–CNT/ITO nanoelectrode using biotin-avidin coupling. It is shown that the PANI-nFe3O4-CNT platform based biosensor can be used to specifically detect bacteria (N. gonorrhoeae) at minute concentration as low as (1×10−19M) indicating high sensitivity within 45s of hybridization time at 298K by differential pulse voltammetry using methylene blue as electroactive indicator. This bacterial sensor has also been tested with 4 positive and 4 negative PCR amplicons of gonorrhoea affected patient samples. The results of these studies have implications towards the fabrication of a handheld device for Neisseria gonorrhoeae detection that may perhaps result in a decrease in the human immunodeficiency virus infections.
[Display omitted]
► PANI–nFe3O4-CNT nanocomposite prepared onto ITO coated glass plate by facile electrochemical synthesis. ► TEM studies show evidence of PANI-nFe3O4-CNT nanocomposite formation with coating of PANI and nFe3O4 onto the CNT. ► 20 mer bases long biotinylated nucleic acid as a model probe for immobilization. ► Very sensitive and specific to detect pathogenic bacteria such as N. gonorrhoeae at low concentration (1×10−19M) displaying high sensitivity. |
doi_str_mv | 10.1016/j.bioelechem.2012.01.005 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1014104469</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1567539412000096</els_id><sourcerecordid>1010490049</sourcerecordid><originalsourceid>FETCH-LOGICAL-c473t-d6f4689528cf138c221584e44717623c3552335c2646546c4aa0e5b28abbe5fe3</originalsourceid><addsrcrecordid>eNqNkUtLxDAUhYMozjj6F6RLN615N7PUwReIbhTchSS9xQxtU5OO6L83Mj6WugjJDd85F85BqCC4IpjI03VlfYAO3DP0FcWEVphUGIsdNCeqVqWQ9Gk3v4WsS8GWfIYOUlpjjBWpxT6aUcqwZEzNkb0zQ8hmLvRjSH6CYuzM1IbYF9YkaIowFGPo3s3gOz9A6WP-CG--gdKZaPMwZINpYyEVWZVFboLoTVc0MIGbfBgO0V5rugRHX_cCPV5ePKyuy9v7q5vV2W3peM2mspEtl2opqHItYcpRSoTiwHlNakmZY0JQxoSjkkvBpePGYBCWKmMtiBbYAp1sfccYXjaQJt375KDrzABhk3ROjhPMuVz-B8V8ifPJqNqiLoaUIrR6jL438T1Dn5zUa_1bhv4sQ2OicxlZevy1ZWN7aH6E3-ln4HwLQI7l1UPUyXkYHDQ-5ux0E_zfWz4A5XGggw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1010490049</pqid></control><display><type>article</type><title>Nanobiocomposite platform based on polyaniline-iron oxide-carbon nanotubes for bacterial detection</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Singh, Renu ; Verma, Rachna ; Sumana, G. ; Srivastava, Avanish Kumar ; Sood, Seema ; Gupta, Rajinder K. ; Malhotra, B.D.</creator><creatorcontrib>Singh, Renu ; Verma, Rachna ; Sumana, G. ; Srivastava, Avanish Kumar ; Sood, Seema ; Gupta, Rajinder K. ; Malhotra, B.D.</creatorcontrib><description>The nanocomposite based on polyaniline (PANI)–iron oxide nanoparticles (nFe3O4) and multi walled carbon-nanotubes (CNT) has been fabricated onto indium tin oxide (ITO) coated glass plate via facile electrochemical synthesis of polyaniline in presence of nFe3O4 (~20nm) and CNT (20–80nm in diameter). The results of transmission electron microscopic studies show evidence of coating of PANI and nFe3O4 onto the CNT. The PANI-nFe3O4–CNT/ITO nanoelectrode has been characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy studies. The biotinylated nucleic acid probe sequence consisting of 20 bases has been immobilized onto PANI–nFe3O4–CNT/ITO nanoelectrode using biotin-avidin coupling. It is shown that the PANI-nFe3O4-CNT platform based biosensor can be used to specifically detect bacteria (N. gonorrhoeae) at minute concentration as low as (1×10−19M) indicating high sensitivity within 45s of hybridization time at 298K by differential pulse voltammetry using methylene blue as electroactive indicator. This bacterial sensor has also been tested with 4 positive and 4 negative PCR amplicons of gonorrhoea affected patient samples. The results of these studies have implications towards the fabrication of a handheld device for Neisseria gonorrhoeae detection that may perhaps result in a decrease in the human immunodeficiency virus infections.
[Display omitted]
► PANI–nFe3O4-CNT nanocomposite prepared onto ITO coated glass plate by facile electrochemical synthesis. ► TEM studies show evidence of PANI-nFe3O4-CNT nanocomposite formation with coating of PANI and nFe3O4 onto the CNT. ► 20 mer bases long biotinylated nucleic acid as a model probe for immobilization. ► Very sensitive and specific to detect pathogenic bacteria such as N. gonorrhoeae at low concentration (1×10−19M) displaying high sensitivity.</description><identifier>ISSN: 1567-5394</identifier><identifier>EISSN: 1878-562X</identifier><identifier>DOI: 10.1016/j.bioelechem.2012.01.005</identifier><identifier>PMID: 22306338</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aniline Compounds - chemistry ; Avidin - chemistry ; Bacteria ; Biosensing Techniques - instrumentation ; Biotin - chemistry ; Electrochemical genosensor ; Electrochemistry - methods ; Ferric Compounds - chemistry ; Gonorrhea - diagnosis ; Gonorrhea - microbiology ; Human immunodeficiency virus ; Humans ; Male ; Microelectrodes ; Microscopy, Electron, Scanning ; Nanocomposite ; Nanocomposites - chemistry ; Nanotubes, Carbon - chemistry ; Neisseria gonorrhoeae ; Neisseria gonorrhoeae - genetics ; Neisseria gonorrhoeae - isolation & purification ; Nucleic acid hybridization ; Nucleic Acid Hybridization - methods ; Sensitivity and Specificity ; Sexually transmitted disease ; Spectroscopy, Fourier Transform Infrared ; Tin Compounds - chemistry ; X-Ray Diffraction</subject><ispartof>Bioelectrochemistry (Amsterdam, Netherlands), 2012-08, Vol.86, p.30-37</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-d6f4689528cf138c221584e44717623c3552335c2646546c4aa0e5b28abbe5fe3</citedby><cites>FETCH-LOGICAL-c473t-d6f4689528cf138c221584e44717623c3552335c2646546c4aa0e5b28abbe5fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bioelechem.2012.01.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22306338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singh, Renu</creatorcontrib><creatorcontrib>Verma, Rachna</creatorcontrib><creatorcontrib>Sumana, G.</creatorcontrib><creatorcontrib>Srivastava, Avanish Kumar</creatorcontrib><creatorcontrib>Sood, Seema</creatorcontrib><creatorcontrib>Gupta, Rajinder K.</creatorcontrib><creatorcontrib>Malhotra, B.D.</creatorcontrib><title>Nanobiocomposite platform based on polyaniline-iron oxide-carbon nanotubes for bacterial detection</title><title>Bioelectrochemistry (Amsterdam, Netherlands)</title><addtitle>Bioelectrochemistry</addtitle><description>The nanocomposite based on polyaniline (PANI)–iron oxide nanoparticles (nFe3O4) and multi walled carbon-nanotubes (CNT) has been fabricated onto indium tin oxide (ITO) coated glass plate via facile electrochemical synthesis of polyaniline in presence of nFe3O4 (~20nm) and CNT (20–80nm in diameter). The results of transmission electron microscopic studies show evidence of coating of PANI and nFe3O4 onto the CNT. The PANI-nFe3O4–CNT/ITO nanoelectrode has been characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy studies. The biotinylated nucleic acid probe sequence consisting of 20 bases has been immobilized onto PANI–nFe3O4–CNT/ITO nanoelectrode using biotin-avidin coupling. It is shown that the PANI-nFe3O4-CNT platform based biosensor can be used to specifically detect bacteria (N. gonorrhoeae) at minute concentration as low as (1×10−19M) indicating high sensitivity within 45s of hybridization time at 298K by differential pulse voltammetry using methylene blue as electroactive indicator. This bacterial sensor has also been tested with 4 positive and 4 negative PCR amplicons of gonorrhoea affected patient samples. The results of these studies have implications towards the fabrication of a handheld device for Neisseria gonorrhoeae detection that may perhaps result in a decrease in the human immunodeficiency virus infections.
[Display omitted]
► PANI–nFe3O4-CNT nanocomposite prepared onto ITO coated glass plate by facile electrochemical synthesis. ► TEM studies show evidence of PANI-nFe3O4-CNT nanocomposite formation with coating of PANI and nFe3O4 onto the CNT. ► 20 mer bases long biotinylated nucleic acid as a model probe for immobilization. ► Very sensitive and specific to detect pathogenic bacteria such as N. gonorrhoeae at low concentration (1×10−19M) displaying high sensitivity.</description><subject>Aniline Compounds - chemistry</subject><subject>Avidin - chemistry</subject><subject>Bacteria</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biotin - chemistry</subject><subject>Electrochemical genosensor</subject><subject>Electrochemistry - methods</subject><subject>Ferric Compounds - chemistry</subject><subject>Gonorrhea - diagnosis</subject><subject>Gonorrhea - microbiology</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Male</subject><subject>Microelectrodes</subject><subject>Microscopy, Electron, Scanning</subject><subject>Nanocomposite</subject><subject>Nanocomposites - chemistry</subject><subject>Nanotubes, Carbon - chemistry</subject><subject>Neisseria gonorrhoeae</subject><subject>Neisseria gonorrhoeae - genetics</subject><subject>Neisseria gonorrhoeae - isolation & purification</subject><subject>Nucleic acid hybridization</subject><subject>Nucleic Acid Hybridization - methods</subject><subject>Sensitivity and Specificity</subject><subject>Sexually transmitted disease</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Tin Compounds - chemistry</subject><subject>X-Ray Diffraction</subject><issn>1567-5394</issn><issn>1878-562X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtLxDAUhYMozjj6F6RLN615N7PUwReIbhTchSS9xQxtU5OO6L83Mj6WugjJDd85F85BqCC4IpjI03VlfYAO3DP0FcWEVphUGIsdNCeqVqWQ9Gk3v4WsS8GWfIYOUlpjjBWpxT6aUcqwZEzNkb0zQ8hmLvRjSH6CYuzM1IbYF9YkaIowFGPo3s3gOz9A6WP-CG--gdKZaPMwZINpYyEVWZVFboLoTVc0MIGbfBgO0V5rugRHX_cCPV5ePKyuy9v7q5vV2W3peM2mspEtl2opqHItYcpRSoTiwHlNakmZY0JQxoSjkkvBpePGYBCWKmMtiBbYAp1sfccYXjaQJt375KDrzABhk3ROjhPMuVz-B8V8ifPJqNqiLoaUIrR6jL438T1Dn5zUa_1bhv4sQ2OicxlZevy1ZWN7aH6E3-ln4HwLQI7l1UPUyXkYHDQ-5ux0E_zfWz4A5XGggw</recordid><startdate>201208</startdate><enddate>201208</enddate><creator>Singh, Renu</creator><creator>Verma, Rachna</creator><creator>Sumana, G.</creator><creator>Srivastava, Avanish Kumar</creator><creator>Sood, Seema</creator><creator>Gupta, Rajinder K.</creator><creator>Malhotra, B.D.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201208</creationdate><title>Nanobiocomposite platform based on polyaniline-iron oxide-carbon nanotubes for bacterial detection</title><author>Singh, Renu ; Verma, Rachna ; Sumana, G. ; Srivastava, Avanish Kumar ; Sood, Seema ; Gupta, Rajinder K. ; Malhotra, B.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-d6f4689528cf138c221584e44717623c3552335c2646546c4aa0e5b28abbe5fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aniline Compounds - chemistry</topic><topic>Avidin - chemistry</topic><topic>Bacteria</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biotin - chemistry</topic><topic>Electrochemical genosensor</topic><topic>Electrochemistry - methods</topic><topic>Ferric Compounds - chemistry</topic><topic>Gonorrhea - diagnosis</topic><topic>Gonorrhea - microbiology</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Male</topic><topic>Microelectrodes</topic><topic>Microscopy, Electron, Scanning</topic><topic>Nanocomposite</topic><topic>Nanocomposites - chemistry</topic><topic>Nanotubes, Carbon - chemistry</topic><topic>Neisseria gonorrhoeae</topic><topic>Neisseria gonorrhoeae - genetics</topic><topic>Neisseria gonorrhoeae - isolation & purification</topic><topic>Nucleic acid hybridization</topic><topic>Nucleic Acid Hybridization - methods</topic><topic>Sensitivity and Specificity</topic><topic>Sexually transmitted disease</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Tin Compounds - chemistry</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, Renu</creatorcontrib><creatorcontrib>Verma, Rachna</creatorcontrib><creatorcontrib>Sumana, G.</creatorcontrib><creatorcontrib>Srivastava, Avanish Kumar</creatorcontrib><creatorcontrib>Sood, Seema</creatorcontrib><creatorcontrib>Gupta, Rajinder K.</creatorcontrib><creatorcontrib>Malhotra, B.D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, Renu</au><au>Verma, Rachna</au><au>Sumana, G.</au><au>Srivastava, Avanish Kumar</au><au>Sood, Seema</au><au>Gupta, Rajinder K.</au><au>Malhotra, B.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanobiocomposite platform based on polyaniline-iron oxide-carbon nanotubes for bacterial detection</atitle><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle><addtitle>Bioelectrochemistry</addtitle><date>2012-08</date><risdate>2012</risdate><volume>86</volume><spage>30</spage><epage>37</epage><pages>30-37</pages><issn>1567-5394</issn><eissn>1878-562X</eissn><abstract>The nanocomposite based on polyaniline (PANI)–iron oxide nanoparticles (nFe3O4) and multi walled carbon-nanotubes (CNT) has been fabricated onto indium tin oxide (ITO) coated glass plate via facile electrochemical synthesis of polyaniline in presence of nFe3O4 (~20nm) and CNT (20–80nm in diameter). The results of transmission electron microscopic studies show evidence of coating of PANI and nFe3O4 onto the CNT. The PANI-nFe3O4–CNT/ITO nanoelectrode has been characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy studies. The biotinylated nucleic acid probe sequence consisting of 20 bases has been immobilized onto PANI–nFe3O4–CNT/ITO nanoelectrode using biotin-avidin coupling. It is shown that the PANI-nFe3O4-CNT platform based biosensor can be used to specifically detect bacteria (N. gonorrhoeae) at minute concentration as low as (1×10−19M) indicating high sensitivity within 45s of hybridization time at 298K by differential pulse voltammetry using methylene blue as electroactive indicator. This bacterial sensor has also been tested with 4 positive and 4 negative PCR amplicons of gonorrhoea affected patient samples. The results of these studies have implications towards the fabrication of a handheld device for Neisseria gonorrhoeae detection that may perhaps result in a decrease in the human immunodeficiency virus infections.
[Display omitted]
► PANI–nFe3O4-CNT nanocomposite prepared onto ITO coated glass plate by facile electrochemical synthesis. ► TEM studies show evidence of PANI-nFe3O4-CNT nanocomposite formation with coating of PANI and nFe3O4 onto the CNT. ► 20 mer bases long biotinylated nucleic acid as a model probe for immobilization. ► Very sensitive and specific to detect pathogenic bacteria such as N. gonorrhoeae at low concentration (1×10−19M) displaying high sensitivity.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22306338</pmid><doi>10.1016/j.bioelechem.2012.01.005</doi><tpages>8</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1567-5394 |
ispartof | Bioelectrochemistry (Amsterdam, Netherlands), 2012-08, Vol.86, p.30-37 |
issn | 1567-5394 1878-562X |
language | eng |
recordid | cdi_proquest_miscellaneous_1014104469 |
source | MEDLINE; Elsevier ScienceDirect Journals Complete |
subjects | Aniline Compounds - chemistry Avidin - chemistry Bacteria Biosensing Techniques - instrumentation Biotin - chemistry Electrochemical genosensor Electrochemistry - methods Ferric Compounds - chemistry Gonorrhea - diagnosis Gonorrhea - microbiology Human immunodeficiency virus Humans Male Microelectrodes Microscopy, Electron, Scanning Nanocomposite Nanocomposites - chemistry Nanotubes, Carbon - chemistry Neisseria gonorrhoeae Neisseria gonorrhoeae - genetics Neisseria gonorrhoeae - isolation & purification Nucleic acid hybridization Nucleic Acid Hybridization - methods Sensitivity and Specificity Sexually transmitted disease Spectroscopy, Fourier Transform Infrared Tin Compounds - chemistry X-Ray Diffraction |
title | Nanobiocomposite platform based on polyaniline-iron oxide-carbon nanotubes for bacterial detection |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T08%3A55%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nanobiocomposite%20platform%20based%20on%20polyaniline-iron%20oxide-carbon%20nanotubes%20for%20bacterial%20detection&rft.jtitle=Bioelectrochemistry%20(Amsterdam,%20Netherlands)&rft.au=Singh,%20Renu&rft.date=2012-08&rft.volume=86&rft.spage=30&rft.epage=37&rft.pages=30-37&rft.issn=1567-5394&rft.eissn=1878-562X&rft_id=info:doi/10.1016/j.bioelechem.2012.01.005&rft_dat=%3Cproquest_cross%3E1010490049%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1010490049&rft_id=info:pmid/22306338&rft_els_id=S1567539412000096&rfr_iscdi=true |