Crystallization of a 79kDa fragment of the hook protein FlgE from Campylobacter jejuni

A 79kDa fragment of the bacterial flagellar hook protein FlgE from Campylobacter jejuni was cloned, overexpressed, purified and crystallized. Two different crystal forms were obtained. Synchrotron X-ray diffraction data showed that the first crystal form, which diffracted to 4.9Aa resolution, belonged to the tetragonal crystal system, with space group I4122 and unit-cell parameters a = b = 186.2, c = 386.6Aa, alpha = beta = gamma = 90 degree . The second crystal form diffracted to 2.5Aa resolution and belonged to the monoclinic crystal system, with space group P21 and unit-cell parameters a = 75.7, b = 173.8, c = 150.8Aa, alpha = gamma = 90, beta = 106.5 degree . SeMet protein was also overexpressed, purified and crystallized, and a 2.6Aa resolution MAD data set was collected.