Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography

Cryoelectron tomography provides unprecedented insights into the macromolecular and supramolecular organization of cells in a close-to-living state. However because of the limited thickness range (

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2012-03, Vol.109 (12), p.4449-4454
Hauptverfasser:	Rigort, Alexander, Bäuerlein, Felix J. B, Villa, Elizabeth, Eibauer, Matthias, Laugks, Tim, Baumeister, Wolfgang, Plitzko, Jürgen M
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological Sciences Cryoelectron Microscopy - methods Cryoultramicrotomy Cytoplasm Dictyostelium Electron microscopes electron microscopy Electrons Equipment Design Eukaryotes eukaryotic cells Eukaryotic Cells - cytology Fourier Analysis Freezing Ice Ion beams Ions Macromolecular Substances - chemistry Microscopy Microscopy, Electron, Scanning - methods Microscopy, Electron, Transmission - methods Nuclear membrane Nuclear pore Prokaryotic cells Protein subunits Temperature Tomography Tomography, X-Ray Computed - methods Vitrification
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Cryoelectron tomography provides unprecedented insights into the macromolecular and supramolecular organization of cells in a close-to-living state. However because of the limited thickness range (
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.1201333109