Horseradish Peroxidase and CD146 Reaction of Deciduous Teeth Pulp Stem Cells

To isolate high-quality human postnatal stem cells from accessible resources is an important goal for stem-cell research. Extracted / exfoliated human deciduous tooth contains multipotent stem cells. Normal deciduous teeth from 6-8 years of age, with more than half the tooth root were extracted in the Pedodontics department. Cells from the enzyme treated pulps were cultured and cells obtained from subsequent healthy colonies were combined for cell surface markers determination process. Cells were positive for CD146 marker, cells also demonstrated horseradish peroxidase (HRP) activity. These findings hold good that dental pulp seem to have derived from neural crest. Key words: Deciduous teeth pulp stem cells, HRP activity, CD146, endothelial progenitor cells, multipotent.