Relationship between abiotic stress and formaldehyde concentration in tissue culture of Datura innoxia Mill

The effect of abiotic stresses such as light, dark conditions and formaldehyde (HCHO) deprivation with dimedone, was investigated on the growth and HCHO level in Datura innoxia Mill. callus cultures. The HCHO as its dimedone adduct form (formaldemethone) was isolated and characterised by overpressured layer chromatography (OPLC). In the cultures cultivated both in dimedone free and dimedone containing medium the endogenous HCHO level was in general higher in light than in the dark. The HCHO deprivation with dimedone did not influence basically the actual endogenous HCHO level in Datura callus tissues, however, the tissue mass was changed.[PUBLICATION ABSTRACT]