An investigation of the role played by the E-4031-sensitive (rapid delayed rectifier) potassium current in isolated rabbit atrioventricular nodal and ventricular myocytes

The aim of this study was to measure and compare the profile of rapid delayed rectifier potassium current (I ^sub Kr^) elicited by action potential (AP) waveforms applied to isolated rabbit atrioventricular nodal (AVN) and ventricular myocytes. All measurements were made using whole-cell patch-clamp recordings at 37°C. In AVN myocytes, I ^sub Kr^ during voltage steps and slow ramp depolarisations showed "inward rectification" (characteristic for this channel) at positive potentials. The E-4031-sensitive current showed half-maximal activation at -10.8±0.86 mV, with a slope factor for the activation relation of 6.5±0.77 mV (n=7). During AVN APs, I ^sub Kr^ rapidly reached a peak after the AP upstroke and remained at similar amplitude until late in AP repolarisation. At the maximum diastolic potential following the AVN AP, a component of I ^sub Kr^ remained which decayed during the pacemaker depolarisation, consistent with a role for the current in generating AVN pacemaker activity. In ventricular myocytes I ^sub Kr^ was small at the beginning of the AP, and increased slowly during the AP plateau. Measurement of Ba-sensitive-inward rectifier K current (I ^sub K1^) in ventricular myocytes revealed that I ^sub K1^ rapidly increased during the final AP repolarisation phase, whilst I ^sub Kr^ declined. It is concluded that I ^sub Kr^ may participate in both AP repolarisation and the pacemaker depolarisation in AVN cells, whilst in ventricular myocytes, I ^sub Kr^ and I ^sub K1^ participate in controlling early and final AP repolarisation respectively.[PUBLICATION ABSTRACT]