Moonlighting Role of a Poly-[gamma]-Glutamate Synthetase Component from Bacillus subtilis: Insight into Novel Extrachromosomal DNA Maintenance

Moonlighting Role of a Poly-[gamma]-Glutamate Synthetase Component from Bacillus subtilis: Insight into Novel Extrachromosomal DNA Maintenance

The Bacillus subtilis structural gene pgsE was investigated as a tool for extrachromosomal DNA maintenance (EDM). It ameliorated the stability of high-copy-number vectors, regardless of whether they were derived from rolling-cycle or theta-mode replicons, without any selective pressure. This unique...

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Veröffentlicht in:Applied and environmental microbiology 2011-04, Vol.77 (8), p.2796
Hauptverfasser: Yamashiro, Daisuke, Minouchi, Yutaka, Ashiuchi, Makoto
Format: Artikel
Sprache:eng
Schlagworte:
Chromosomes
Deoxyribonucleic acid
DNA
Gene expression
Gram-positive bacteria
Microbiology
Protein synthesis
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Zusammenfassung:The Bacillus subtilis structural gene pgsE was investigated as a tool for extrachromosomal DNA maintenance (EDM). It ameliorated the stability of high-copy-number vectors, regardless of whether they were derived from rolling-cycle or theta-mode replicons, without any selective pressure. This unique EDM phenomenon may occur via a trans-acting mechanism. [PUBLICATION ABSTRACT]
ISSN:0099-2240
1098-5336