POTENTIAL ANTICANCER COMPOUND FROM CERBERA ODOLLAM

The cytotoxicity of the leaf of Cerbera odollam was investigated against two breast cancer cell lines (T47D and MCF7), two ovarian cancer cell lines (SKOV3 and CaOV3) and a normal (Vero) cell line. The crude methanolic extract was subjected to liquid—liquid fractionation with ethyl acetate, butanol and water. Ethyl acetate fraction exhibited higher cytotoxic effect than methanol, butanol and aqueous fractions. Following bioassay guided isolation, 17βH-neriifolin was isolated as a potential anticancer agent from C. odollam leaf. It showed potent anticancer activity with IC50 values of 17, 21, 28, 32 and 24 nM against MCF7, T47D, SKOV3, CaOV3 and Vero cell lines respectively. Sitotoksisiti daun Cerbera odollam dikaji terhadap dua titisan sel barah payudara (T47D dan MCF7), dua titisan sel barah ovari (SKOV3 and CaOV3) dan satu titisan sel normal (Vero). Ekstrak metanol C. odollam dipisahkan melalui pemeringkatan cecair—cecair dengan etil asetat, butanol dan air. Pecahan etil asetat menunjukkan kesan sitotoksisiti yang lebih kuat daripada pecahan metanol, butanol dan air. Melalui pemencilan berpandukan bioasai, 17βH-neriifolin yang berpotensi sebagai agen antibarah diasingkan daripada daun C. odollam. Ia menunjukkan aktiviti antibarah yang kuat dengan nilai IC50 17 nM, 21 nM, 28 nM, 32 nM dan 24 nM masing-masing terhadap titisan-titisan sel MCF7, T47D, SKOV3, CaOV3 dan Vero.