A polycationic antimicrobial and biocompatible hydrogel with microbe membrane suctioning ability

Despite advanced sterilization and aseptic techniques, infections associated with medical implants have not been eradicated. Most present coatings cannot simultaneously fulfil the requirements of antibacterial and antifungal activity as well as biocompatibility and reusability. Here, we report an antimicrobial hydrogel based on dimethyldecylammonium chitosan (with high quaternization)- graft -poly(ethylene glycol) methacrylate (DMDC-Q- g -EM) and poly(ethylene glycol) diacrylate, which has excellent antimicrobial efficacy against Pseudomonas aeruginosa , Escherichia coli , Staphylococcus aureus and Fusarium solani . The proposed mechanism of the antimicrobial activity of the polycationic hydrogel is by attraction of sections of anionic microbial membrane into the internal nanopores of the hydrogel, like an ‘anion sponge’, leading to microbial membrane disruption and then microbe death. We have also demonstrated a thin uniform adherent coating of the hydrogel by simple ultraviolet immobilization. An animal study shows that DMDC-Q- g -EM hydrogel coating is biocompatible with rabbit conjunctiva and has no toxicity to the epithelial cells or the underlying stroma. A polymeric hydrogel coating shows impressive antimicrobial activity against both bacteria and fungi. The biocompatible and reusable coating, formed of a polycationic nanoporous hydrogel, is thought to act by drawing anionic sections of phospholipids on bacterial cell membranes into its pores, causing membrane disruption and cell death.