The RET/PTC3 oncogene activates classical NF-[kappa]B by stabilizing NIK

The oncogenic fusion protein RET/PTC3 (RP3) that is expressed in papillary thyroid carcinoma (PTC) and thyroid epithelia in Hashimoto's thyroiditis activates nuclear factor-kappa B (NF-[kappa]B) and induces pro-inflammatory gene expression; however, the mechanism of this activation is unknown....

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Veröffentlicht in:Oncogene 2011-01, Vol.30 (1), p.87
Hauptverfasser: Neely, R J, Brose, M S, Gray, C M, Mccorkell, K A, Leibowitz, J M, Ma, C, Rothstein, J L, May, M J
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container_issue 1
container_start_page 87
container_title Oncogene
container_volume 30
creator Neely, R J
Brose, M S
Gray, C M
Mccorkell, K A
Leibowitz, J M
Ma, C
Rothstein, J L
May, M J
description The oncogenic fusion protein RET/PTC3 (RP3) that is expressed in papillary thyroid carcinoma (PTC) and thyroid epithelia in Hashimoto's thyroiditis activates nuclear factor-kappa B (NF-[kappa]B) and induces pro-inflammatory gene expression; however, the mechanism of this activation is unknown. To address this, we expressed RP3 in murine embryonic fibroblasts (MEFs) lacking key classical and noncanonical NF-[kappa]B signaling components. In wild-type MEFs, RP3 upregulated CCL2, CXCL1, granulocyte-macrophage colony-stimulating factor and tumor necrosis factor expression and activated classical but not noncanonical NF-[kappa]B. RP3-activated NF-[kappa]B in I[kappa]B kinase (IKK)[beta]-/- MEFs but not IKK[alpha]- or NF-[kappa]B essential modulator (NEMO)-deficient cells and activation was inhibited by a peptide that blocks NEMO binding to the IKKs. RP3 increased the levels of NF-[kappa]B-inducing kinase (NIK) and did not activate NF-[kappa]B in NIK-deficient MEFs. Notably, NIK stabilization was not accompanied by TRAF3 degradation demonstrating that RP3 disrupts normal basal NIK regulation. Dominant-negative NIK blocked RP3-induced NF-[kappa]B activation and an RP3 signaling mutant (RP3Y588F) did not stabilize NIK. Finally, examination of PTC specimens revealed strong positive staining for NIK. We therefore conclude that RP3 activates classical NF-[kappa]B via NIK, NEMO and IKK[alpha]. Importantly, our findings reveal a novel mechanism for oncogene-induced NF-[kappa]B activation via stabilization of NIK. [PUBLICATION ABSTRACT]
doi_str_mv 10.1038/onc.2010.396
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To address this, we expressed RP3 in murine embryonic fibroblasts (MEFs) lacking key classical and noncanonical NF-[kappa]B signaling components. In wild-type MEFs, RP3 upregulated CCL2, CXCL1, granulocyte-macrophage colony-stimulating factor and tumor necrosis factor expression and activated classical but not noncanonical NF-[kappa]B. RP3-activated NF-[kappa]B in I[kappa]B kinase (IKK)[beta]-/- MEFs but not IKK[alpha]- or NF-[kappa]B essential modulator (NEMO)-deficient cells and activation was inhibited by a peptide that blocks NEMO binding to the IKKs. RP3 increased the levels of NF-[kappa]B-inducing kinase (NIK) and did not activate NF-[kappa]B in NIK-deficient MEFs. Notably, NIK stabilization was not accompanied by TRAF3 degradation demonstrating that RP3 disrupts normal basal NIK regulation. Dominant-negative NIK blocked RP3-induced NF-[kappa]B activation and an RP3 signaling mutant (RP3Y588F) did not stabilize NIK. Finally, examination of PTC specimens revealed strong positive staining for NIK. We therefore conclude that RP3 activates classical NF-[kappa]B via NIK, NEMO and IKK[alpha]. Importantly, our findings reveal a novel mechanism for oncogene-induced NF-[kappa]B activation via stabilization of NIK. 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subjects Cellular biology
Gene expression
Oncology
Rodents
Thyroid diseases
title The RET/PTC3 oncogene activates classical NF-[kappa]B by stabilizing NIK
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