Intracellular energetics and critical PO^sub 2^ in resting ischemic human skeletal muscle in vivo

During ischemia and some types of muscular contractions, oxygen tension (...) declines to the point that mitochondrial ATP synthesis becomes limited by oxygen availability. Although this critical ... has been determined in animal tissue in vitro and in situ, there remains controversy concerning potential disparities between values measured in vivo and ex vivo. To address this issue, we used concurrent heteronuclear magnetic resonance spectroscopy (MRS) to determine the critical intracellular ... in resting human skeletal muscle in vivo. We interleaved measurements of deoxymyoglobin using ...-MRS with measures of highenergy phosphates and pH using ...-MRS, during 15 min of ischemia in the tibialis anterior muscles of 6 young men. ATP production and intramyocellular ... were quantified throughout ischemia. Critical ..., determined as the ... corresponding to the point where PCr begins to decline (...) in resting muscle during ischemia, was 0.35 ± 0.20 Torr, means ± SD. This in vivo value is consistent with reported values ex vivo and does not support the notion that critical ... in resting muscle is higher when measured in vivo. Furthermore, we observed a 4.5-fold range of critical ... values among the individuals studied. Regression analyses revealed that time to ... was associated with critical ... and the rate of myoglobin desaturation (r = 0.83, P = 0.04) but not the rate of ATP consumption during ischemia. The apparent dissociation between ATP demand and myoglobin deoxygenation during ischemia suggests that some degree of uncoupling between intracellular energetics and oxygenation is a potentially important factor that influences critical ... in vivo. (ProQuest: ... denotes formulae/symbols omitted.)