Role of T cells in the adjuvant effect ofbacillus firmus on the immune system of mice: Intranasal and intratracheal immunization study with ovalbumin

Functions of T cells were determined after intranasal and intratracheal immunization of mice with ovalbumin (Ova) andBacillus firmus (Bf), a Gram-positive nonpathogenic bacterium of the external environment, or delipidatedBf (dBf) as adjuvants, with the aim to elucidate the mechanism of support of Ova-specific antibody production caused byBf that had been observed in an identical experiment. NeitherBf nor dBf in a mixture with Ova stimulated Ova-specific T-cell response tested as antigen-specific blast transformation. By contrast, a mild polyclonal stimulation was observed in splenocytes from mice given dBf. In vitro incubation of splenocytes with 100 µg (but not 10 µg) ofBf or dBf led to a highly significant inhibition of proliferation below the control level in all groups of animals. Supernatants of splenocyte cultures were further tested for cytokine production. IL-10 and IFN-γ were released afterin vitro challenge with dBf and in some cases also withBf. Analysis of sera demonstrated that administration of Ova + adjuvant brought about an increase in anti-Ova IgG1, IgG2a and IgG2b whereas treatment with Ova alone caused a rise in IgG1 only. The role ofBf or dBf in the enhancement of antigen-specific antibody production could be in influencing macrophages and inducing cytokine milieu composed of IL-10, IFN-γ and other factors that leads to a bystander stimulation of specifically activated Ova-B cell receptor (Ova-BCR)-bearing cells.