Construction of promoter-probe shuttle vectors forEscherichia coli and corynebacteria on the basis of promoterless α-amylase gene

Construction of promoter-probe shuttle vectors forEscherichia coli and corynebacteria on the basis of promoterless α-amylase gene

We constructed new promoter-probe vectors forE. coli and corynebacteria based on the promoterless α-amylase gene originating fromBacillus subtilis. Vectors pJUPAE1 and pJUPAE2 are suitable for isolation of transcriptionally active fragments from plasmids, phages or genomic DNA α-Amylase activity can...

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Veröffentlicht in:Folia microbiologica 2000-04, Vol.45 (2), p.114-120
Hauptverfasser: Ugorčáková, J., Bukovská, G., Timko, J.
Format: Artikel
Sprache:eng
Schlagworte:
Amylases
Carbohydrates
Construction
Phages
Plasmids
Shuttle vectors
Substrates
α-Amylase
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Zusammenfassung:We constructed new promoter-probe vectors forE. coli and corynebacteria based on the promoterless α-amylase gene originating fromBacillus subtilis. Vectors pJUPAE1 and pJUPAE2 are suitable for isolation of transcriptionally active fragments from plasmids, phages or genomic DNA α-Amylase activity can be easily visually detected on agar plates containing a chromogenic substrate, or by direct measurement of α-amylase activity.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02817408