Calculations on O2 transfer in capsules with animal cells for the determination of maximum capsule size without O2 limitation

The size of the capsules without O2 limitation and maximum allowable capsule size for different cell densities were calculated by the observable modulus (modified Thiele modulus). When hybridoma (ATCC HB-8852) at 2.5 3 × 105cells/ ml was encapsulated and grown, the cell density reached to 2.6 × 107c...

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Veröffentlicht in:	Biotechnology letters 1998-06, Vol.20 (6), p.549-552
Hauptverfasser:	KIM, S.-K, YU, S.-H, SON, J.-H, HÜBNER, H, BUCHHOLZ, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Bacteria Biological and medical sciences Biotechnology Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microbiology
Online-Zugang:	Volltext
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Zusammenfassung:	The size of the capsules without O2 limitation and maximum allowable capsule size for different cell densities were calculated by the observable modulus (modified Thiele modulus). When hybridoma (ATCC HB-8852) at 2.5 3 × 105cells/ ml was encapsulated and grown, the cell density reached to 2.6 × 107cells/ml in the 7th day of incubation. The average diameter of the capsule was 2.1mm. The maximum cell density obtained from experiment agreed with the calculated cell density for the given size of the capsules. © Rapid Science Ltd. 1998[PUBLICATION ABSTRACT]
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1005341526365