Enzymatic synthesis of two novel non-reducing oligosaccharides using transfructosylation activity with [beta]-fructofuranosidase from Arthrobacter globiformis

Two novel non-reducing oligosaccharides together with tri- and tetra-saccharides were synthesized by transfructosylation activity from sucrose as a donor and cellobiose or cellotriose as an acceptor with a purified β-fructofuranosidase from Arthrobacter globiformis IFO 3062, and these oligosaccharides were identified as O-β-d-glucopyranosyl-(1\to4)-α-d-glucopyranosyl-(1\to2)-α,β-d-fructofuranoside and O-β-d-glucopyranosyl-(1\to4)-β-d-glucopyranosyl-(1\to4)-α-d-glucopyranosyl-(1\to2)-α, β-d-fructofuranoside by spectrometric analyses. Both oligosaccharides were stable under condition at 100°C for 30 min, and showed no degradation at pH 2.[PUBLICATION ABSTRACT]