Inhibition of the growth of human hepatoma cell line bothin vitro andin vivo by transducing CKI genep21 WAF-1 with GE7 targeting gene delivery system

The EGF receptor-mediated targeting gene delivery system GE7 was used to transduce exogenous genepCEP-p21 ^sup ^sup WAF-1^^ into human hepatocellular carcinoma cell bothin vitro andin vivo. Afterin vitro transduction of the exogenous gene, the growth of the cell lines SMMC-7721 and BEL-7402 was significantly inhibited compared with the control. On day 8 the inhibition rates of the above cell lines reached 56.0% and 66.7%, respectively. Thein vivo experiment showed that the growth of human hepatoma transplanted in nude mice injected with GE7 gene delivery system subcutaneously once a week for 3 weeks was remarkably inhibited compared with that of untransfected control. The average tumor weight of the experiment group was (0.083 ±0.043) g, while that of the control group was (0.281 ±0.173) g. The difference is significant (P