A New Benzofuran from the Roots of Eupatorium chinense L. and Its α-glucosidase and PTP1B Inhibitory Activities

A new benzofuran, named eupbenzofuran A (1), along with four known compounds, 10,11-dihydroxy-10,11-dihydroeuparin (2), 5-[1'-hydroxyethyl]-2-1'-hydroxyisoprolyl]-benzofuran (3), 3α,6-dihydroxytremetone (4), odoratin (5) were isolated from the roots of Eupatorium chinenseL. The structure of compounds were identified by NMR, MS, CD and other spectroscopic methods and comparisons with relevant literature data. Compound 1 had favorable dual inhibitory activities against α-glucosidase and protein tyrosine phosphatase 1B (PTP1B). However, compounds 2-5 had no significant inhibitory activities effects on α-glucosidase and PTP1B (IC50＞50 μg/mL). Molecular docking technique was used to calculate the interaction of compound 1 with α-glucosidase and PTP1B, respectively, and the calculated results showed that compound 1 had both strong binding to α-glucosidase and PTP1B.