Shedding light on some dark branches: The under‐appreciated diversity of gymnosome pteropods and their coiled thecosome prey in the Neotropics

Holoplanktonic organisms are thought to have enormous populations sizes, expansive geographic ranges, and low species diversity. Previous work suggests that pelagic gastropods are no exception to this generalization. However, most of these data are derived from temperate species, whereas the bulk of pteropod diversity occurs in the tropics. Here, we present DNA barcode data for COI focused on the under‐studied limacinoid, gymnosome, and pseudothecosome pteropods collected from the coastal waters of Panama. We applied four molecular species delimitation approaches to determine the number of operational taxonomic units (OTUs) identifiable from our sequences and all data available from Barcode of Life Database (BOLD) and GenBank and compared these to the Barcode Index Number generated by the BOLD. Assemble Species by Automatic Partitioning (ASAP), Poisson Tree Processes (PTP), Bayesian Poisson Tree Processes (bPTP), and Generalized Mixed Yule Coalescent (GMYC) methods generally gave congruent results and suggest that pteropod diversity in the tropics is significantly underrepresented both in DNA sequence databases and in the number of named OTUs. Of 42 specimens collected and sequenced, we recovered 16 OTUs, only one of which belonged to an OTU already represented in sequence databases. Neighbor‐joining trees including the previously published sequences show that in all three groups morphospecies can include genetically divergent lineages. Many very divergent (>15% from nearest neighbor) taxa are also still represented by only a single sequence, suggesting that there is a large amount of cryptic or pseudo‐cryptic diversity still to be described. To aid this future endeavor, we include some preliminary 16S data derived from new pteropod‐specific primers. Resumen Se cree que los organismos holoplanctónicos poseen poblaciones numerosas, rangos geográficos expansivos y baja diversidad de especies. Trabajos previos sugieren que los gasterópodos pelágicos no son una excepción a esta generalización; sin embargo, la mayoría de estos datos provienen de especies en áreas templadas, mientras que la mayor diversidad de pterópodos ocurre en los trópicos. En este artículo se proporcionan datos de códigos de barras de ADN del COI, enfocados en los poco estudiados pterópodos Limacinoidea, Gymnosomata y Pseudothecosomata recolectados en las aguas costeras de Panamá. El análisis de estas secuencias, combinado con datos disponibles de BOLD y GenBank, sugiere que la diversidad e