ReLo: Analyse direkter Protein-Protein-Interaktionen durch Relokalisation

ReLo: Analyse direkter Protein-Protein-Interaktionen durch Relokalisation

We have developed a cell-based relocalization (ReLo) assay for the detection of direct protein-protein interactions. In the assay, one protein is anchored to a membrane, causing the other protein to relocalize upon interaction. The assay can be used to study the influence of protein domains, post-tr...

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Veröffentlicht in:Biospektrum 2024-11, Vol.30 (7), p.769-771
Hauptverfasser: Miederer, Levi, Jeske, Mandy
Format: Artikel
Sprache:ger
Schlagworte:
Biochemistry
Biomedical and Life Sciences
Fluorescence microscopy
Human Genetics
Life Sciences
Microbiology
Pharmacology/Toxicology
Post-translation
Protein interaction
Transfection
Wissenschaft Special
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Jeske, Mandy
description We have developed a cell-based relocalization (ReLo) assay for the detection of direct protein-protein interactions. In the assay, one protein is anchored to a membrane, causing the other protein to relocalize upon interaction. The assay can be used to study the influence of protein domains, post-translational modifications, protein conformations, and effector molecules on interactions. The ReLo assay requires plasmid transfection and fluorescence microscopy and can handle structurally complex proteins.
doi_str_mv 10.1007/s12268-024-2355-y
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subjects Biochemistry
Biomedical and Life Sciences
Fluorescence microscopy
Human Genetics
Life Sciences
Microbiology
Pharmacology/Toxicology
Post-translation
Protein interaction
Transfection
Wissenschaft Special
title ReLo: Analyse direkter Protein-Protein-Interaktionen durch Relokalisation
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