ReLo: Analyse direkter Protein-Protein-Interaktionen durch Relokalisation

We have developed a cell-based relocalization (ReLo) assay for the detection of direct protein-protein interactions. In the assay, one protein is anchored to a membrane, causing the other protein to relocalize upon interaction. The assay can be used to study the influence of protein domains, post-translational modifications, protein conformations, and effector molecules on interactions. The ReLo assay requires plasmid transfection and fluorescence microscopy and can handle structurally complex proteins.