Catechin as a functional additive in electrospun PCL/gelatin/nHA nanocomposite fibers for tissue engineering applications

The promise of polymeric nanocomposite fibers in the biomedical field is well‐documented due to their adjustable properties and versatility. Electrospun fiber mats, which mimic the extracellular matrix, are particularly noted for their potential in tissue regeneration and repair. In this study, we investigate the role of catechin, a flavonoid, in enhancing cellular response to electrospun nanocomposite fibers of polycaprolactone (PCL), gelatin, and nanohydroxyapatite (nHA). Nanocomposite fibers were fabricated with varying concentrations of catechin and comprehensive analyses of the composite fibers were performed to evaluate their structural and chemical properties. In vitro, assays were performed to examine cell viability and ascertain the influence of catechin on cellular responses to these nanocomposite fibers. Simultaneously, in vivo studies assessed tissue responses to these materials. The findings revealed that incorporating catechin into PCL/gelatin/nHA nanocomposite fibers improved cellular responses in vitro as demonstrated by enhanced cell viability. Furthermore, in vivo investigations displayed positive tissue responses with these fibers, indicating their capacity to enhance cell growth and accelerate tissue regeneration. This study elucidates the potential of catechin as an integral component in designing polymeric nanocomposite fibers, potentially broadening the scope of their biomedical applications. PCL/gelatin/nHA/catechin nanocomposite fibers for tissue engineering applications.